陕西科技大学学报（自然科学版）2012，Vol.30Issue(6)：71-74,4.
Plackett-Burman法筛选保加利亚乳杆菌发酵培养基氮源
Screening nitrogen source of fermentation medium of Lactobacillus bulgaricus by Plackett-Burman design
摘要
Abstract
As one of the yogurt fermentation bacterias-Lactobacillus bulgaricus , of which enrichment medium components,was optimized after examined seven kinds of nitrogens which affected its growth. Based on the cheap and readily available raw materials, the important factors that influnce on the growth of Lactobacillus bulgaricus were filtered with Plackett-Burman (PB) experimental design method. The results showed that three kinds of nitrogen sources of newborn calf serum(Ρ = 0. 025 3) , yeast extract(Ρ = 0. 070 7) and casein hydroly-zate (Ρ = 0. 093 9)had a significant affect on the growth of Lactobacillus bulgaricus , which showed significant differences in more than 90% probability level. What's more, newborn calf serum and yeast extract showed a negative effect, the casein hydrolyzate had a positive effect. The other factors did not show significant differences in more than 90% probability level. So we chosed the three substances as the multiplication factors of Lactobacillus bulgaricus. The trials initially identified the amount of three kinds of nitrogen sources in order to laid the foundation for further optimization of medium for the future.关键词
保加利亚乳杆菌/培养基/氮源/Plackett-Burman设计Key words
Lactobacillus bulgaricus / medium/ nitrogen sources/ Plackett-Burman design分类
轻工纺织引用本文复制引用
陈合,李串娜,舒国伟..Plackett-Burman法筛选保加利亚乳杆菌发酵培养基氮源[J].陕西科技大学学报（自然科学版）,2012,30(6):71-74,4.基金项目
陕西省重大科技创新项目(2011ZKC11-1) （2011ZKC11-1）
陕西省科技厅工业攻关项目(2012K08-17) （2012K08-17）
