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α-环糊精葡萄糖基转移酶菌株的筛选与鉴定

张智维 雷新辉 张海群

陕西科技大学学报(自然科学版)2012,Vol.30Issue(6):89-92,4.
陕西科技大学学报(自然科学版)2012,Vol.30Issue(6):89-92,4.

α-环糊精葡萄糖基转移酶菌株的筛选与鉴定

Selecting and identification of the producing strain for α-Cyclodextrin Glycosyltransferase

张智维 1雷新辉 1张海群1

作者信息

  • 1. 陕西科技大学生命科学与工程学院,陕西西安710021
  • 折叠

摘要

Abstract

22 strains of bacteria which could yield α-CGTase were prescreened from soil simples. These soils are rich in starch. 3 strains of them with higher enzymatic activity were further selected by assaying transparent zone. Finally, we obtained a strain with activity of 0. 86 U/mL from setting up a standard curve of enzymatic activity. This research showed that N1 was a Klebsiella oxytoca based on its colony morphology, microscopic observation and physiology and biochemical characteristic.

关键词

α-环糊精葡萄糖基转移酶/筛选/鉴定/酶活力

Key words

α-Cyclodextrin Glyeosyltransferase/ selection/ identification/ enzyme activity

分类

生物科学

引用本文复制引用

张智维,雷新辉,张海群..α-环糊精葡萄糖基转移酶菌株的筛选与鉴定[J].陕西科技大学学报(自然科学版),2012,30(6):89-92,4.

基金项目

陕西省自然基金项目(2009JM2005) (2009JM2005)

陕西省教育厅专项科研计划项目(09JK372) (09JK372)

陕西科技大学学报(自然科学版)

OACSTPCD

2096-398X

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