新疆农业科学2012，Vol.49Issue(12)：2239-2244,6.DOI:10.6048/j.issn.1001-4330.2012.12.014
石河子地区棉田棉铃虫种群体内相关酶活及含量的测定
Determination of Contents and Activities of Detoxification Enzymes of Helicoverpa armigera in Field Populations in Shihezi District
摘要
Abstract
[Objective] In order to provide experimental data for the research on the insecticide resistance of H. armigera, the contents and activities of the main detoxification enzymes such as carboxylesterase ( CarE ) , phosphatase, glutathione S - transferase ( GST) , monooxygenase ( MFOs ) of H. armigera in field populations in Shihezi district were determined when compared with the susceptible strain of H. armigera. [ Method ] The enzyme protein contents and specific activities were measured by ultraviolet spectrophotometry. [Result] The content of MFOs of H. armigera in field populations in Shihezi district was 105.943 167μg/insect, distinctly higher than that of susceptible strain which was 96. 158 47 μg/insect. Furthermore, the specific activity of the former was 0.485 3 ±0.002 1 μM/μg·30 min, also higher than that of susceptible strain which was 0. 352 3 ±0.001 4 μM/μg·30 min. There were no significant differences of the contents and activities of the other three enzymes between field populations in Shihezi district and the susceptible strain. [ Conclusion ] The increased activity of MFOs was one of the main factors contributing to the enhanced resistance to insecticides of H. armigera in field populations in Shihezi district.n field ppulations关键词
棉铃虫/抗药性/解毒酶Key words
H.armigera/ insecticide resistance/ detoxification enzyme分类
农业科技引用本文复制引用
汪小东,武万锋,张建华,赵伊英..石河子地区棉田棉铃虫种群体内相关酶活及含量的测定[J].新疆农业科学,2012,49(12):2239-2244,6.基金项目
石河子大学自然科学研究项目(ZRKX2009YB08) （ZRKX2009YB08）
新疆生产建设兵团科技攻关计划项目(2010KJ06) （2010KJ06）
