新疆农业科学2012，Vol.49Issue(12)：2263-2268,6.DOI:10.6048/j.issn.1001-4330.2012.12.018
盐穗木HcPS_H基因真核表达载体的构建及转化
Construction and Transformation of Eukaryotic Express Vector of HcPS_ H Gene from Halostachys caspica
摘要
Abstract
[Objective] Halostachys caspica is a leaf - succulent euhalophytic plant capable of surviving under high salinity, and a genus of Chenopodiaceae plants, widely distributed in Xinjiang arid or semi - arid desert saline environments. PS H gene is a part of photosynthetic response to light system gene, which plays an important role in Photosynthesis in plants. [Method] It is helpful to learn the function of HcPS _H in salt tolerance procession of Halostachys caspica by constructing the eukaryotic express system of this gene. We cloned the gene of HcPS _ H from Halostachys caspica by PCR. The target gene fragments and the plasmid pREP -5N were digested with the restriction enzymes SaiI and NotI, and then were linked together by the T4 DNA ligase. Finally, the recombinant eukaryotic expression plasmid HcPS_H-5N was obtained. [Result] The identification results of the individual bacterial colonies PCR, double enzymes digestion and sequencing showed that the recombinant expressing plasmid HcPS _ H - 5N was constructed successfully. [ Conclusion] The recombinant plasmid was transformed into the yeast competent cells by electro - transformation, and the yeast single colonies PCR indicted that the recombinant expressing plasmid HcPS _ H - 5N was successfully transferred.关键词
盐穗木/HcPS_H基因/真核表达载体/重组质粒构建Key words
Halostachys caspica/ HcPS _ H gene/ Eukaryotic expression vector/ construction of recombi-nant plasmid分类
农业科技引用本文复制引用
郝晓燕,张毓露,足木热木,刘小利,黄全生..盐穗木HcPS_H基因真核表达载体的构建及转化[J].新疆农业科学,2012,49(12):2263-2268,6.基金项目
新疆维吾尔自治区农作物生物技术重点实验室开放课题"盐穗木耐盐基因的克隆与功能鉴定"(XJDX0201-2011-05) （XJDX0201-2011-05）
新疆农业科学院优秀青年科技人才基金"盐生植物盐角草耐盐基因的发掘与功能研究"(XJNKY-2012-003) （XJNKY-2012-003）
