畜牧兽医学报2013，Vol.44Issue(1)：49-56,8.
鸭Mef2bnb基因的克隆及其mRNA丰度在肌肉组织中的发育性表达变化
Molecule Cloning and Developmental Expression of Mef2bnb Gene in Muscle Tissues from Duck
摘要
Abstract
This experiment was conducted to obtain and analyze cDNA sequence of duck Mef2bnb (Myocyte enhancer factor 2B neighbor), and to investigate the developmental expression of Mef2bnb in duck muscle tissues. The full-length Me/2bnb cDNA was cloned from leg muscle of duck by using RACE method. Meanwhile, real-time quantitative PCR method was used to analyze Mef2bnb gene developmental expression in muscle tissues of duck, which from embryo ten days to one week after birth. The results showed that full sequence of duck Mef2bnb gene was 935 base-pairs(bp) including a 5'untranslated region(UTR) of 51 bp and 3'UTR of 518 bp, a complete open reading frame (ORF)of 366 bp encoding 121 amino acid (aa) residues. The structural analysis indicates that there was no signal peptide in this peptide chain, and the amino acid from 1 to 117 was a NEP domain which was conservative between the Mef2bnb of duck and other animals. Moreover, the different expression levels of Mef2bnb were found in chest muscle, leg muscle and heart tissue. The expression of Mef2bnb in chest muscle was higher than that in leg muscle at the same stage. It was deduced that duck Mef2bnb may have the function enhancing fiber Ⅱ formation, besides, the high expression in heart implied that Mef2bnb might relate to the process of heart development. Therefore,the results of this work may lay the foundation for further study on the structure and the role of Mef2bnb gene in muscle tissues in duck.关键词
鸭/Mef2bnb/克隆/序列分析/发育性表达Key words
duck/ Mef2bnb/ cloning/ sequence analysis/ developmental expression分类
农业科技引用本文复制引用
王浩瀚,刘贺贺,孙玲利,张荣萍,李欣欣,杨超,李亮,王继文..鸭Mef2bnb基因的克隆及其mRNA丰度在肌肉组织中的发育性表达变化[J].畜牧兽医学报,2013,44(1):49-56,8.基金项目
国家现代农业水禽产业技术体系(CARS-43-6) （CARS-43-6）
