畜牧兽医学报2013，Vol.44Issue(1)：102-107,6.
雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化
Gene Expression Analysis of CD8α and Its Signaling Pathway Related Genes Treated with DHV in Duckling
摘要
Abstract
This experiment was conducted to explore gene expression of CD8α and its signaling pathway related genes after virus infection. The experimental animal model of infected duck hepatitis virus (DHV) was constructed by using DHV to infect 3-day-old JinDing ducklings without maternal antibody. Then the gene expressions of CD8α, MHC Ⅰ, MHC Ⅱ and TNFα in some tissue of different groups of DHV were detected by RT-qPCR. The specific band of conservative regions in the DHV 3D gene was amplified in morbid group and non-morbid group. This outcome showed that the infected duck hepatitis virus animal model of CD8α gene was constructed successfully. The results of RT-qPCR demonstrated that the CD8α, MHC Ⅰ, MHC Ⅱ genes were up-regulated in non-morbid group and down-regulated in morbid group of all tissue in different degrees, while the TNF-α gene were up-regulated in morbid group of Liver, spleen, lung, and kidney. The research showed that the expression of the CD8α gene and related gene of its pathway was altered after duck hepatitis virus infection. The results contribute to a better understanding of the duck CD8α gene regulatory role in cellular immunity.关键词
鸭/CD8α基因/雏鸭肝炎病毒/基因表达Key words
duck/CD8a gene/DHV/gene expression分类
农业科技引用本文复制引用
徐琪,陈国宏,陈阳,黄正洋,童一宇,荣光辉,赵文明,张扬,李秀,段修军..雏鸭肝炎病毒感染后鸭CD8α基因及其通路相关基因表达变化[J].畜牧兽医学报,2013,44(1):102-107,6.基金项目
国家自然科学基金项目(31101704) （31101704）
现代农业产业技术体系建设专项资金(CARS-43-3) （CARS-43-3）
江苏高校优势学科建设工程资助项目(苏政办发[2011]137号) （苏政办发[2011]137号）
