畜牧兽医学报2013，Vol.44Issue(1)：115-121,7.
淫羊藿多糖脂质体对鸡淋巴细胞增殖及IL-2、IL-4和IFN-γ mRNA表达的影响
Effect of Epimedium Polysaccharide Liposome on Lymphocyte Proliferation,mRNA Expression of IL-2, IL-4 and IFN-γ in Chicken
摘要
Abstract
In order to study the action mechanism of epimedium polysaccharide liposome (EPSL) on immunological enhancement activity, the effects of EPSL on lymphocyte proliferation and mRNA expression of IL-2, IL-4 and IFN-γ were determined by MTT method and real-time fluorescent quantitation PCR assay, taking epimedium polysaccharide (EPS) as control. The results showed that EPSL could significantly enhance lymphocyte proliferation, and at 31. 250 μg · mL-1 and 3. 906 μg · mL-1, EPSL singly or synergistically with PHA could stimulate the lymphocyte proliferation which was better than EPS. And EPSL also could increase mRNA expression of IL-2, IL-4 and IFN-γ, and were significantly better than EPS at 62. 500 and 31. 250 μg · mL-1. At 62. 500 μg · mL-1, the effect of EPSL was the strongest, at 31. 250 μg · mL-1 being stronger and in a dose-dependant manner. Effect of EPSL on lymphocyte proliferation, mRNA expression of IL-2, IL-4 and IFN-γ in chicken after EPS was encapsulated with liposome关键词
淫羊藿多糖脂质体/鸡/增强免疫/淋巴细胞增殖/细胞因子Key words
epimedium polysaccharide liposome/ chicken/ immune-enhancing/ lymphocyte proliferation/ cytokine分类
农业科技引用本文复制引用
高焕,王德云,郭利伟,张欣兴,赵晓娟,赵晓娜..淫羊藿多糖脂质体对鸡淋巴细胞增殖及IL-2、IL-4和IFN-γ mRNA表达的影响[J].畜牧兽医学报,2013,44(1):115-121,7.基金项目
国家自然科学基金项目(30901085) （30901085）
教育部科学技术重点项目(108153) （108153）
中央高校基本科研业务费专项资金项目(KYZ201117) （KYZ201117）
