畜牧兽医学报2013，Vol.44Issue(1)：145-151,7.
牦牛源鲍氏志贺菌的鉴定及对BALB/c小鼠的致病性
Identification of Shigella boydii in Yak and Its Pathogenicity to BALB/c Mice
摘要
Abstract
The aim of this study is to identify Shigella isolated from healthy slaughter yak, and to determine its pathogenicity to mice. The isolate was identified and eventually characterized by using several methods, including morphology observation, culture characteristics, biochemical tests, serotyping, 16S rRNA sequencing, electron microscopy and evaluation of pathogenicity to BALB/c mice. The results showed that morphological, culture and biochemical characteristics of the isolate is consistent with that of Shigella. The isolate was serotyped as Shigella boydii type 6. Phylogeny analysis based on 16S rRNA indicated that the isolate has close relationship with Shigella boydii and Shigella sonnei in human, and has further relationship with the Shigella from other animals. The isolate is pathogenic to BALB/c mice, and the LD50 is 2. 5×1075CFU. The bacterium is not resistant to antibiotics commonly used. We isolated Shigella boydii type 6 from yak for the first time. The isolate is highly pathogenic and phylogentically related to human Shigella, indicating important significance of yak Shigella in public health.关键词
牦牛/鲍氏6型志贺菌/鉴定/致病性Key words
yak/ Shigella boydii type 6/ identification/ pathogenicity分类
农业科技引用本文复制引用
冉丹丹,于学辉,汤承,李键,宋定州..牦牛源鲍氏志贺菌的鉴定及对BALB/c小鼠的致病性[J].畜牧兽医学报,2013,44(1):145-151,7.基金项目
"十二五"国家科技支撑计划(2012BAD136B06) （2012BAD136B06）
国家公益性行业(农业)科研专项经费(201203009) （农业）
中央高校基本科研业务费科研平台建设项目(11NPT03) （11NPT03）
