畜牧与兽医2013，Vol.45Issue(3)：13-16,4.
抗菌肽PR39在大肠杆菌中的表达及抑菌活性检测
Biological characteristics of the antibacterial peptide PR39 expressed in Escherichia coli
摘要
Abstract
According to the antibacterial peptide PR39 gene sequence in GenBank, a pair of primers was designed and synthesized. The PR39 gene segment was cloned to the express vector pCold, and thus the recombinant plasmid pCold-PR39 was constructed. Then the recom-binant plasmid was transferred into Escherichia coli BL21 (DE3). The analysis of SDS-PAGE and Western blotting showed that after induced with isopropyl β-D-1-thiogalactopyranoside (IPTG) , the target fusion protein was successfully expressed and identified in inclusion bodies. The protein had a molecular weight of 54 ku. The expressed PR39 protein showed obvious antibacterial activity. The in vitro antibacterial test showed that the maximum diameters of inhibition zones of recombinant antibacterial peptide PR39 against E. coli CVCC10141 and Salmonella typhimurium CVCC79102 were 5.7 mm and 4.9 mm, respectively. There was no antibacterial activity against Staphylococcus aureus CVCC25923. This study provides a basis for the application of PR39.关键词
猪抗菌肽PR39/原核表达/抑菌活性Key words
antibacterial peptide PR39/ prokaryotic expression/ antibacterial activity分类
农业科技引用本文复制引用
刘阳欣,李海滨,姜艳平,崔文,乔薪瑗,葛俊伟,李一经,唐丽杰..抗菌肽PR39在大肠杆菌中的表达及抑菌活性检测[J].畜牧与兽医,2013,45(3):13-16,4.基金项目
国家自然科学基金(31072057,30871809) （31072057,30871809）
黑龙江省教育厅创新团队基金(2011TD001). （2011TD001）
