新医学2012，Vol.43Issue(11)：787-791,5.DOI:10.3969/g.issn.0253-9802.2012.11.010
PC-3悬浮成球细胞中ABCG2表达及其对化学治疗耐药性的实验研究
Experimental studies on the ABCG2 expression and chemoresistance of suspension sphere-forming PC-3 cells
摘要
Abstract
Objective: To explore the ABCG2 expression and the chemoresistance of human prostate cancer sphere-forming PC-3 cells. Methods: PC-3 cells were suspension cultured in vitro with a serum-free medium ( SFM) . The expression of ABCG2 mRNA in sphere-forming PC-3 cells was detected with Real-time quantitative PCR (qRT-PCR) . The sensitivity of sphere-forming PC-3 cells to cisplatin was determined with MTT. Results; The PC-3 sphere-forming cells could survive in SFM and form floating cell spheres. The relative expression quantity of ABCG2 mRNA in the sphere-forming cells was 33. 98-fold higher than that of the PC-3 adherent cells ( P < 0. 05). The IC50 of the sphere-forming cells was 4.26-fold higher than that of the PC-3 adherent cells (P <0.05). Conclusion: The ABCG2 expression of humanprostate cancer sphere-forming PC-3 cells is high, and the chemore-sistance of PC-3 sphere-forming cells is very strong.关键词
前列腺癌/悬浮成球细胞/ATP结合盒膜转运蛋白2/化学治疗耐药性Key words
Prostate cancer/ Floating sphere-forming cells/ ABCG2/ Chemoresistance引用本文复制引用
张波,范新兰,林天歆,许可慰,黄海,黄健..PC-3悬浮成球细胞中ABCG2表达及其对化学治疗耐药性的实验研究[J].新医学,2012,43(11):787-791,5.基金项目
国家自然科学基金项目(30500507,30672092) （30500507,30672092）
