烟草科技Issue(1)：58-63,6.
超声提取-GC/MS法测定烟叶中的烷烃
Determination of Alkanes in Tobacco with Ultrasonic Extraction-GC/MS
摘要
Abstract
In order to investigate the distribution and content of alkanes in tobacco, the alkanes in tobacco were extracted respectively by Soxhlet extraction, ultrasonic extraction, simultaneous distillation extraction and steam distillation extraction. The extracts were concentrated and then purified by SPE-Si prior to GC/MS analysis. Ultrasonic extraction was finally chosen for the further study by balancing extraction time and the extraction yield of alkanes. The extraction parameters were optimized by single factor experiments and orthogonal experiment. The results showed that: 1) The optimal parameters were: dichloromethane as solvent, ultrasonic power 560 W, extraction time 15 minutes, sample/solvent ratio 1:10, and repeated extraction for four times. The number of extraction repetition was the key factor affecting extraction yield. 2) Forty-one kinds of alkanes were identified by GC/MS analysis, among them, n-, i-, ai-alkanes between C25-C33 accounted for 99% of the total alkanes. This method is simple, fast and sensitive, features good reproducibility, and is suitable for the determination of alkanes in tobacco leaves.关键词
烟叶/烷烃/超声提取/气相色谱/质谱法(GC/MS)Key words
Tobacco leaf/ Alkane/ Ultrasonic extraction/ Gas chromatography/mass spectrometry (GC/MS)分类
轻工纺织引用本文复制引用
刘菲,赵明月,余祥英,曾世通,胡军..超声提取-GC/MS法测定烟叶中的烷烃[J].烟草科技,2013,(1):58-63,6.基金项目
国家烟草专卖局卷烟增香保润重大专项项目"烟叶表面性状及化学成分与保润性能关系研究"(422012AA0370). （422012AA0370）
