烟台大学学报（自然科学与工程版）2013，Vol.26Issue(1)：53-57,5.
壳聚糖酶产生菌筛选及产酶培养优化
Strain Screening and Optimization of Fermentation Conditions for Producing Chitosanase
摘要
Abstract
A high-yield strain of chitosanase is screened from the soil samples collected from Zhifudao ( Yantai, China) , which is obtained with colloidal chitosan as only C-source and determination of chitsanase activity, and then identified as Streptomyces albus. By optimization of medium compositions and culture conditions for chitosanase production, the optimal concentration for higher production of chitosanase are determined as follows; 55 g·L-1 glucose, 0. 5 g·L-1 chitosan, 1 g·L-1 peptone,2 g·L-1 (NH4)2SO4, 8 g·L-1 urea,1g·L-1 NH4CI, 3 g·L-1 KH2PO4, 1 g·L-1 FeSO4·7H2O, 2 g·L-1 ZnSO4·7H2O, 2 g· L-1 CaCl2·6H2O, 2 g·L-1 MnSO4·H2O and 2 g·L-1 NaCl. The maximum activity of chitosanase obtained is 51. 65 U·mL-1 at 28℃, pH 7. 0, 2% inoculum and 50 mL fermentation medium in Erlenmeyer flask (500 mL) for 48 h.关键词
壳聚糖酶/菌株筛选/白色链霉菌/发酵优化Key words
chitosanase/ strain screening/ Streptomyces albus/ fermentation optimization分类
生物科学引用本文复制引用
程仕伟,杨立红,洪波,缪静..壳聚糖酶产生菌筛选及产酶培养优化[J].烟台大学学报（自然科学与工程版）,2013,26(1):53-57,5.基金项目
山东省高等学校科技计划项目(J12LD02),鲁东大学科研基金资助项目(LY20083305). （J12LD02）
