医药导报2012，Vol.31Issue(12)：1616-1618,3.DOI:10.3870/yydb.2012.12.029
浙贝乙素胆酸盐中浙贝乙素和胆酸的含量测定
Determination of Verticinone and Cholic Acid in Verticinone Cholic Acid Salt by HPLCELSD
摘要
Abstract
Objective To establish an HPLC method for determination of verticinone and cholic acid in verticinone-cholic acid salt. Methods The separation was performed on a C18column (250 mm×4. 6 mm,5 μm). The mobile phase was methanol : ammonium acetate (0. 1 mol·L-1) : triethylamine : ethanoic acid (78 :22 : 0. 2:0. 03). The flow rate was 1 mL·min-1. The gas flow rate of ELSD was 2 mL·min-1 and the temperature of drift tube was 72. 1℃. Results The linear ranges of verticinone and cholic acid were 50.4-151. 1 μg·mL-1 (r = 0. 999 6, n = 5) and 50. 5-151.4 μg·mL-1 (r= 0.9999,n = 5) respectively. The average recoveries of the two components were 99. 37% ( RSD = 0. 63% ,n = 9) and 99. 17% (RSD = 0.62% ,n = 9) respectively. Conclusion The method is simple and reliable for the quality control of verticinone and cholicacid salt.关键词
浙贝乙素胆酸盐/浙贝乙素/胆酸/高效液相-蒸发光散射/含量测定Key words
Verticinone-cholic acid salt/Verticinone/Cholic acid/HPLC-ELSD/Content determination分类
医药卫生引用本文复制引用
李娟,张鹏,钟淑文,皮慧芳,阮汉利,吴继洲..浙贝乙素胆酸盐中浙贝乙素和胆酸的含量测定[J].医药导报,2012,31(12):1616-1618,3.基金项目
湖北省自然科学基金资助项目(2008CDA046) （2008CDA046）
