摘要
Abstract
Objective To establish an ultra fast liquid chromatography ( UFLC ) method for determining five saponins in sanqi capsule.Methods Chromatography was performed on an shimadzu C18 column (75 mm×2.0 mm, 2.2 μm) at 25℃.The gradient elution was acetonitrile-water at a flow rate of 0.3 mL·min-1.The detection wavelength was at 203 nm.Results The linear ranges for notoginsenoside R,,ginsenoside Rg,,ginsenoside Re, ginsenoside Rb, and ginsenoside Rd were 9.38-351.62 ng (R2 = 1), 52.11-1 954.12 ng (R2=0.999 9), 6.85-152.17 ng (R2=0.999 6), 44.99-1 687.25 ng (R2= 1),9.96-221.27 ng (R2= 1),respectively.The average recoveries were 96.6% for notoginsenoside R1( RSD = 0.9% ),97.1% for ginsenoside Rg1(RSD= 1.4%),101.0% for ginsenoside Re ( RSD= 1.4% ), 97.9% for ginsenoside Rb1 ( RSD = 2.4% ),98.2% for ginsenoside Rd (RSD= 1.8%), respectively (n= 6).Conclusion The method is simple, rapid and suitable for determination of five saponins in sanqi capsule.关键词
三七胶囊/三七皂苷R1/人参皂苷Rg1/人参皂苷Re/人参皂苷Rb1/人参皂苷Rd/液相色谱,超快速Key words
Sanqi capsule/Notoginsenoside R1/Ginsenoside Rg1/Ginsenoside Re/Ginsenoside Rb1/Ginsenoside Rd/UFLC分类
医药卫生
