湖北医药学院学报2012，Vol.31Issue(6)：453-457,5.
核酸内切酶制备的siRNA抑制HBV基因表达和复制
Endoribonuclease-prepared siRNAs Inhibit Hepatitis B Virus Gene Expression and Replication
摘要
Abstract
Objective To investigate the inhibition on HBV gene expression and replication by endoribonuclease-prepared siRNAs (esiRNAs). Methods EsiRNAs targeting the HBsAg (SeiRNA) and HBcAg (CesiRNA) were prepared and co-transfected into HepG2 cells with a replication-competent HBV construct ( pHY106 + wta). The HBV gene expression and replication were determined by chemiluminescent microparticle imrnunoassay,Northern and Southern blotting,respectively. Results EsiRNAs inhibited the HBV gene expression and replication specifically and dose dependent])-. At concentration of 100 nM,about 75.96% and 68. 09% of HBsAg,79. 69% and 78. 40% of HBeAg,60% and 58% of HBV mRNA,and 90% and 87% of HBV replicative intermediates were inhibited by SesiRNA and CesiRNA,respectively. Moreover,SesiRNA maintained the inhibitory effect on various natural occurred HBs mutants. Conclusion EsiRNAs strongly inhibit HBV gene expression and replication and may have an advantage against HBV strains which are variable genetically.关键词
乙型肝炎病毒/RNA干扰/小干扰性RNA/核酸内切酶制备的siRNAKey words
Hepatitis B virus, HBV/ RNA interference, RNAi/ small interference RNA, siRNA/ Endoribonuclease-prepared siRNA,esiRNA分类
生物科学引用本文复制引用
孟忠吉,张永红,汤守兵,柯昌征,李东,陈悦..核酸内切酶制备的siRNA抑制HBV基因表达和复制[J].湖北医药学院学报,2012,31(6):453-457,5.基金项目
湖北省自然科学基金(2010CDZ036),湖北医药学院优秀中青年科技创新团队项目(2011CXX02) （2010CDZ036）
