中国病理生理杂志Issue(1)：20-27,8.DOI:10.3969/j.issn.1000-4718.2013.01.004
西洋参茎叶总皂苷通过抑制过度内质网应激减轻大鼠心肌缺血/再灌注损伤
Panax quinquefolium saponins protect rat myocardium from ischemia/reperfusion injury through attenuating excessive endoplasmic reticulum stress
摘要
Abstract
AIM: To investigate whether excessive endoplasmic reticulum stress (ERS) is involved in the protective mechanism of Panax quinquefolium saponins (PQS) against ischemia/reperfusion (I/R) injury in rat myocardium. METHODS: The model of myocardial I/R injury in vivo was made by ligating the left anterior descending artery for 45 min followed by 24 h of reperfusion in SD rats. The hemodynamics and serum content of cardiac troponin T ( cTnT) were measured. The myocardial infarct size was measured by Evans blue and 2,3, 5-triphenyltetrazolium chloride (TTC) staining. Cardiomyocyte apoptosis was detected using in situ TDT-mediated dUTP nick end labeling (TUNEL). The protein levels of glucose-regulated protein 78 (GRP78) , calreticulin (CRT) , C/EBP homologous protein (CHOP) , caspase-12, apopto-sis-associated proteins Bax and Bcl-2 were determined by Western blotting. RESULTS: Compared with I/R group, the mean arterial pressure in PQR + IR group was decreased by 32. 0% , and left ventricular ± dp/dtmax was increased by 64. 0% and 35. 0% , respectively. The serum content of cTnT was decreased by 53. 3% , the percentage of area of necrosis ( AN)/area at risk (AAR) was reduced by 65.5% and the apoptosis rate was decreased by 54.9%. The myocardial pathological changes were improved. Bcl-2 protein expression was increased by 110. 0% and that of Bax was decreased by 47. 8%. CRT protein expression was decreased by 43. 4 % , CHOP protein expression and the protein level of cleaved caspase-12 were decreased by 38.6% and 23.7% in PQS + I/R group. CONCLUSION: PQS alleviates I/R injury in myocardium by inhibition of excessive ERS.关键词
西洋参茎叶总皂苷/缺血/再灌注/内质网应激Key words
Panax quinquefolium saponins/ Ischemia/reperfusion/ Endoplasmic reticulum stress分类
医药卫生引用本文复制引用
王琛,刘蜜,孙胜,宋丹丹,刘秀华,史大卓..西洋参茎叶总皂苷通过抑制过度内质网应激减轻大鼠心肌缺血/再灌注损伤[J].中国病理生理杂志,2013,(1):20-27,8.基金项目
科学技术部国际科技合作项目资助课题(No.2010DFA31690) （No.2010DFA31690）
