中国病理生理杂志Issue(1)：116-120,5.DOI:10.3969/j.issn.1000-4718.2013.01.020
液体复苏联合氢气吸入对脓毒性休克大鼠肺脏的作用
Effects of early liquid resuscitation combined with hydrogen inhalation on lung injury in septic shock rats induced by lipopolysaccharides
摘要
Abstract
AIM: To study the effects of fluid resuscitation combined with 2% hydrogen inhalation on lung injury in septic shock rats induced by lipopolysaccharides (LPS). METHODS: Sixty male Wistar rats were randomized to 4 groups: normal control group, septic shock control group, fluid resuscitation group and fluid resuscitation plus hydrogen inhalation group. Liquid resuscitation was applied to the rats in fluid resuscitation group and fluid resuscitation plus hydrogen inhalation group. The rats in fluid resuscitation plus hydrogen inhalation group also received 2% hydrogen inhalation and the rats in other groups inhaled air for control. The pathological changes of the lung were observed under light microscope. The levels of inflammatory mediators in bronchoalveolar lavage fluid (BALF) were measured by ELISA. The expression of Fas and Bcl-2 was detected by Western blotting. RESULTS: Compared with fluid resuscitation group, the levels of inflammatory mediators in BALF and the expression of Fas in lung tissues were decreased, and the expression of Bcl-2 in lung tissue was increased in fluid resuscitation plus hydrogen inhalation group. CONCLUSION: Fluid resuscitation with 2% hydrogen inhalation exhibits a protective effect on lung injury induced by LPS.关键词
脓毒症/肺损伤/液体复苏/氢气Key words
Sepsis/ Lung injury/ Fluid resuscitation/ Hydrogen分类
医药卫生引用本文复制引用
刘伟,孙裕强,孙宁,刘志..液体复苏联合氢气吸入对脓毒性休克大鼠肺脏的作用[J].中国病理生理杂志,2013,(1):116-120,5.基金项目
国家自然科学基金资助项目(No.81101415) （No.81101415）
