中国病理生理杂志Issue(1)：150-154,5.DOI:10.3969/j.issn.1000-4718.2013.01.026
皖南地区眼镜蛇毒活性组分镇痛效应的初步研究
Analgesic effect of active component from crude cobra venom in Wannan area
摘要
Abstract
AIM: To explore the analgesic effect of active component from cobra venom in Wannan area. METHODS: The active component was purified from crude venom of cobra by cation-exchange chromatography and size-exclusion chromatography. The purity and molecular weight of the active component were determined by SDS-polyacrylam-ide gel electrophoresis and capillary zone electrophoresis. The analgesic activity was analyzed by hot-plate test and acetic acid writhing test. Normal saline and morphine were used as controls. RESULTS: A single active component was obtained from crude cobra venom. The molecular weight was about 6 500 D. The analgesic effect of the active component persisted from 0.5 h to 8 h after hot-plate test. With intraperitoneal injection of the active component at doses of 0.03 mg/kg, 0. 1 mg/kg and 0.3 mg/kg in mice, the writhing inhibition rates were 27% , 50% and 70% , respectively. No statistical difference between 0.3 mg/kg group and morphine group was observed. CONCLUSION; The active component from crude cobra venom has a dose-dependent analgesic effect.关键词
眼镜蛇毒/镇痛Key words
Cobra venom/ Analgesia分类
医药卫生引用本文复制引用
闵志雪,黄璐,孙瑶,包鹏举,王海华,张根葆..皖南地区眼镜蛇毒活性组分镇痛效应的初步研究[J].中国病理生理杂志,2013,(1):150-154,5.基金项目
安徽省科技厅科技项目(No.10021303009) （No.10021303009）
皖南医学院重点科研项目培育基金(No.WK22012Z01) （No.WK22012Z01）
