中国病理生理杂志2013，Vol.29Issue(2)：225-230,6.DOI:10.3969/j.issn.1000-4718.2013.02.007
类风湿关节炎滑膜血管内皮细胞中Smoothened的表达及其意义
Expression of Smoothened and its role in endothelial cells in synovial tissues of rheumatoid arthritis
摘要
Abstract
AIM: To investigate the expression of Sonic Hedgehog signaling pathway-associated factor Smoothened ( Smo) and its role in endothelial cells in synovial tissue of active rheumatoid arthritis ( RA) . METHODS: Smo expression in synovial tissue from 4 RA patients and 4 patients with trauma or meniscal injury ( without arthritis, used for control) was detected by the method of immunohistochemistry. Human umbilical vein endothelial cell line EA. hy926 was used as the model of synovial vascular endothelial cells. The expression of Smo was detected by Western blotting after TNF-α treatment. The small interfering RNA (siRNA) specifically targeting Smo gene was synthesized and transfected into EA. hy926 cells. The interference efficiency of the siRNA on the production of Smo protein was determined by Western blotting. The cells were treated with TNF-a and actinomycin D (ActD) 24 h after siRNA transfection. The cell survival rate was determined by CCK-8 assay and the apoptotic rate was examined by flow cytometry. RESULTS; Smo was highly expressed in synovial tissue from active RA patients, especially in endothelial cells as compared with control group. TNF-a significantly increased the protein expression of Smo in EA. hy926 cells. EA. hy926 cells transfected with Smo-siRNA showed a significant decrease in the cell viability with the cell survival rate of (24.30 ±0.45)% and the apoptotic rate of (48. 00 ±1.96)% , as compared with those in negative control group [(36.86 ±0.62)% and (31.70 ±0.82)% , respectively] . CONCLUSION: Smo may play a role in the regulation of apoptosis in endothelial cells in RA synovium.关键词
关节炎,类风湿/Smoothened/RNA干扰/内皮细胞/细胞凋亡Key words
Arthritis, rheumatoid/ Smoothened/ RNA interference/ Endothelial cells/ Apoptosis分类
医药卫生引用本文复制引用
朱尚玲,彭蔚湘,罗敏琪,王明霞,林灼锋,黄建林..类风湿关节炎滑膜血管内皮细胞中Smoothened的表达及其意义[J].中国病理生理杂志,2013,29(2):225-230,6.基金项目
国家自然科学基金资助项目(No.81072480) （No.81072480）
广东省自然科学基金资助项目(No.10151008901000210) （No.10151008901000210）
