中国病理生理杂志2013，Vol.29Issue(2)：302-307,6.DOI:10.3969/j.issn.1000-4718.2013.02.020
雷奈酸锶通过TGF-β1/Smad通路促进骨髓间充质干细胞向成骨细胞分化
Strontium ranelate promotes osteogenic differentiation of rat bone mesenchymal stem cells through TGF-β1/Smad signaling pathway
摘要
Abstract
AIM: To study the roles of transforming growth factor β1 (TGF-β1 )/Smad signaling pathway in strontium ranelate ( Sr) -induced osteogenic differentiation of rat bone marrow mesenchymal stem cells ( BMSCs). METHODS: In the process of osteogenic differentiation of rat BMSCs, the expression of phosphorylated Smad2 (p-Smad2) and Runx2 was detected by Western blotting after the cells were treated with Sr. BMSCs were pretreated with SB431542, a selective inhibitor of TGF-β1 , or Smad2 small interfering RNA (Smad2-siRNA) , followed by Sr treatment, and then the expression of p-Smad2 and Runx2 was observed. At the same time, the activity of alkaline phosphatase (ALP) and the level of calcium nodules were detected to determine the osteogenic differentiation of BMSCs. RESULTS: The expression levels of p-Smad2 and Runx2 were enhanced under the action of Sr in the process of osteogenic differentiation of rat BMSCs. The expression of p-Smad2 reached to maximum when BMSCs were treated with Sr at concentration of 1 mmol/L for 1 h. The expression of Runx2 reached to maximum when BMSCs were treated with Sr at concentration of 1 mmol/L for 5 d. The pre-treatment with SB431542 or Smad2-siRNA inhibited not only the expression of p-Smad2 and Runx2, but also the activity of ALP and the level of calcium nodules. CONCLUSION: Sr promotes the osteogenic differentiation of rat BMSCs through the TGF-β1/Smad signaling pathway.关键词
雷奈酸锶/骨髓间充质干细胞/转化生长因子β/Smad2蛋白/Runx2蛋白Key words
Strontium ranelate/ Bone marrow mesenchymal stem cells/ Transforming growth factor beta/ Smad2 protein/ Runx2 protein分类
医药卫生引用本文复制引用
黄晓丹,吕辉珍,靳思思,郭润民,吴文..雷奈酸锶通过TGF-β1/Smad通路促进骨髓间充质干细胞向成骨细胞分化[J].中国病理生理杂志,2013,29(2):302-307,6.基金项目
广东省自然科学基金资助项目(No.S2012010009403) （No.S2012010009403）
广东省科技计划项目(No.2011B031800002) （No.2011B031800002）
广州市科技计划项目(No.2012J4300082) （No.2012J4300082）
