中国病理生理杂志2013，Vol.29Issue(2)：314-319,6.DOI:10.3969/j.issn.1000-4718.2013.02.022
高原鼠兔血红素氧合酶1的基因克隆及序列分析
Cloning and sequencing of heme oxygenase-1 gene from Ochotona curzo-niae
摘要
Abstract
AIM: To clone and analyze the heme oxygenase-1 (HO-1) gene from Ochotona curzoniae (plateau pika) . METHODS: The cDNA of HO-1 was cloned by RT-PCR and rapid ampliation of cDNA ends (RACE) from the liver of Ochotona curzoniae. The bioinformatic analysis of HO-1 gene was performed. RESULTS; The cDNA of HO-1 gene in Ochotona curzoniae was obtained. The data of the sequence was deposited into GenBank and the accession number is JX035934. The full length of cDNA was 1 466 bp, including 873 bp encoding sequence (290 amino acids). Homology comparison showed that the DNA sequence of the HO-1 gene was highly homologous with Oryctolagus cuniculus (89% ) , Homo sapiens (87% ) , Bos taurus (85% ) , Mus musculus (79% ) , Rattus norvegicus (84% ) , Sus scrqfa (85% ) and Equus caballus (85% ) , and the amino acid sequence of HO-1 was identified with the homology of 89% , 85% , 84% , 80% , 79% , 82% and 67% , respectively. Phylogenetic analysis suggested that the structure of HO-1 was highly similar to that in Oryctolagus cuniculus. CONCLUSION: The HO-1 gene of Ochotona curzoniae was successfully cloned and provides essential information for elucidating the possible roles of HO-1 in adapting of Ochotona curzoniae to extremely high altitude.关键词
高原鼠兔/血红素氧合酶1/低氧适应/cDNA克隆/序列分析Key words
Ochotona curzoniae/ Heme oxygenase-1/ Hypoxia adaptation/ cDNA cloning/ Sequence analysis分类
医药卫生引用本文复制引用
杜杨,杨应忠,马燕,曹雪峰,格日力..高原鼠兔血红素氧合酶1的基因克隆及序列分析[J].中国病理生理杂志,2013,29(2):314-319,6.基金项目
国家重点基础研究发展规划项目(973)(No.2012CB518200) （973）
国家国际合作项目(No.2011DFA32720) （No.2011DFA32720）
国家自然科学基金资助项目(No.31160232 （）
No.30393133) （）
青海省自然科学基金资助项目(No.2011-Z-919Q) （No.2011-Z-919Q）
