中国草食动物科学2013，Vol.33Issue(1)：17-20,4.
云南半细毛羊成纤维细胞系建立及其生物学特性分析
Establishment and the Biological Characteristics of Fibroblast Cell Line in Yunnan Semi-fine Wool Sheep
摘要
Abstract
In this study, the fibroblasts cell line derived from ear marginal tissue of Yunnan semi-fine wool sheep was successfully established using the primary explants technique and cryopreservation technology. The morphology, dynamic growth and karyotypes of the cultured cells after different passages were analyzed. The results showed that the population doubling times (PDT) of passage 7,12 and 17 were 26,42,and 47 h respectively. The growth curve of the cultured cells appeared "S shape. The ratio of established fibroblast cells with normal diploid (2n=54) were 93%, 86% and 81% following 7,12 and 17 passages. The total and average relative length of chromosome set were 197.86 and 3.66, respectively.关键词
云南半细毛羊/成纤维细胞系/生长曲线/生物学特性Key words
Yunnan semi-fine wool sheep/fibroblast cell line/growth curve/karyotype分类
农业科技引用本文复制引用
吴帅帅,李东江,吕春荣,权国波,郭成裕,洪琼花..云南半细毛羊成纤维细胞系建立及其生物学特性分析[J].中国草食动物科学,2013,33(1):17-20,4.基金项目
国家绒毛羊体系建设项目(CARS-40) （CARS-40）
家养动物平台(NIAGR) （NIAGR）
