中国当代儿科杂志2013，Vol.15Issue(2)：91-94,4.DOI:10.7499/j.issn.1008-8830.2013.02.004
糖皮质激素对慢性免疫性血小板减少症患儿树突状细胞的影响
Effect of glucocorticoid on dendritic cells in children with chronic immune thrombocytopenia
摘要
Abstract
Objective To investigate the change in dendritic cells (DCs) in children with chronic immune thrombocytopenia (cITP) and the effect of glucocorticoid on DCs in children with cITP. Methods Fifteen children with cITP and 20 healthy controls were included in the study. Flow cytometry was used to measure the DC subsets count in the 15 children with cITP before and after glucocorticoid treatment as well as the corresponding values in the 20 healthy controls. The DCs derived from peripheral blood monocytes in children with cITP were cultured in vitro and collected, and their immunophenotypes were determined by flow cytometry. Results Before glucocorticoid treatment, the children with cITP showed no notable change in the absolute count of myeloid DCs (mDCs) but showed decreased absolute count of plasmacytoid DCs ( pDCs) and increased mDC/pDC ratio compared with the healthy controls (P < 0. 05 ). After glucocorticoid treatment, the children with cITP demonstrated increased absolute count of pDCs and decreased absolute count of mDCs and mDC/pDC ratio compared with before treatment (P < 0. 05). Before glucocorticoid treatment, the children with cITP had significantly higher positive rates of HLA-DR, CD80, CD83 and CD86 on peripheral blood DCs than the healthy controls ( P < 0. 01). All the positive rates were significantly decreased after glucocorticoid treatment (P <0. 01) , so that there was no significant difference from the healthy controls ( P > 0. 05 ) . Conclusions Disproportion and functional disturbance of DC subsets is associated with the pathogenesis of cITP in children. Glucocorticoid can strengthen the immunosuppression of DCs in children with cITP, which may contribute to the effectiveness of glucocorticoid as a treatment.关键词
免疫性血小板减少症/树突状细胞/糖皮质激素/儿童Key words
Immune thrombocytopenia/ Dendritic cell/ Glucocorticoid/ Child引用本文复制引用
旷文勇,顾艳艳,郑敏翠,张广森,宋国才,李婉丽,杨海霞,蒋小梅,张本山,吴攀..糖皮质激素对慢性免疫性血小板减少症患儿树突状细胞的影响[J].中国当代儿科杂志,2013,15(2):91-94,4.基金项目
湖南省科技厅博士后资助专项(No.2010RS4028) （No.2010RS4028）
湖南省卫生厅计划项目(No.B2007158). （No.B2007158）
