中国动脉硬化杂志2013，Vol.21Issue(1)：32-36,5.
血管紧张素Ⅱ和氧化型低密度脂蛋白对大鼠血管平滑肌细胞Smurf2和Ⅰ型胶原表达的影响
Effects of Angiotensin Ⅱ and Oxidized Low Density Lipoprotein on Smurf2 and Collagen Ⅰ Expression in Cultured Rat Vascular Smooth Muscle Cells
摘要
Abstract
Aim To investigate angiotensin Ⅱ (Ang Ⅱ ) and oxidized low density lipoprotein (ox-LDL) regulating the expression of Smurf2 and collagen Ⅰ in cultured rat vascular smooth muscle cells ( VSMC ). Methods The mRNA expression was tested by RT-PCR. The protein expression was examined by Western blot. The protein expression of collagen Ⅰ was examined by ELISA. Results Compared with control group, Ang Ⅱ decreased SmurC expression ( P ＜ 0. 05) and enhanced collagen Ⅰ expression ( P ＜ 0. 05 ) , ox-LDL enhanced Smurf2 expression ( P ＜ 0. 01 ) and decreased collagen I expression ( P ＜ 0. 05 ). Ox-LDL concentration-dependently induced Ang Ⅱ -decreased SmurO expression (P ＜ 0. 05 ) and collagen I concentration. Conclusion Ang Ⅱ decreased SmurO expression in cultured VSMC. Ang Ⅱ may inhibit SmurG expression to improve collagen Ⅰ production. ox-LDL enhanced Smurf2 expression. ox-LDL may improve the expression of SmurC to suppress the production of collagen Ⅰ . ox-LDL concentration-dependently induced Ang Ⅱ -decreased SmurG expression, while decreased collagen Ⅰ expression.lagen Ⅰ productio关键词
Smurf2/血管紧张素Ⅱ/氧化型低密度脂蛋白/Ⅰ型胶原Key words
SmurG/ Oxidized Low Density Lipoprotein/ Angiotensin Ⅱ / Collagen Ⅰ / Vascular Smooth Muscle Cell分类
医药卫生引用本文复制引用
刘怡菲,刘先哲..血管紧张素Ⅱ和氧化型低密度脂蛋白对大鼠血管平滑肌细胞Smurf2和Ⅰ型胶原表达的影响[J].中国动脉硬化杂志,2013,21(1):32-36,5.基金项目
三峡大学硕士学位论文培优基金资助(2011PY032) （2011PY032）
