中国感染控制杂志2012，Vol.11Issue(6)：417-421,5.
ICU分离的多重耐药鲍曼不动杆菌PFGE分型及Ⅰ型整合子介导的耐药研究
PFGE analysis and class Ⅰ integron-mediated resistance investigation of multidrug-resistant Acinetobacter baumannii from an intensive care unit
摘要
Abstract
Objective To investigate the positive rate of class I integron in Acinetobacter baumannii (AB) isolated from an intensive care unit (ICU), analyze the homology of multidrug-resistant AB (MDR-AB) , and explore the molecular mechanisms of class I integron involved in drug resistance and the spread. Methods Forty MDR-AB isolates from the ICU between January 2008 and December 2009 were collected, drug resistance of all isolates were detected by Kirby-Bauer method, PCR and DNA sequencing were undertaken to clarify the context of gene cassette. Pulsed-field gel electrophoresis was used to analyze the homology of MDR-AB. Results Class I integron was detected in 75. 00% (30/40) of all isolates, 2 types of gene cassettes were identified, namely aacAA-catB8-aad A1 and aacC1 -orfX-orfX-orfX'-aadA1. Forty isolates were classified into 4 distinct genotypes by PFGE analysis,26 isolates belonged to clone B that were predominant epidemic strain. Conclusion class I integrons are widespread in MDR-AB, and spread horizontally in ICU. The epidemic of AB in ICU maybe caused by MDR-AB carrying aacAA-catB8-aadA1 cassette.关键词
鲍曼不动杆菌/抗药性,微生物/多重耐药/脉冲场凝胶电泳/重症监护室/医院感染Key words
Acinetobacter baumannii/ drug resistance, microbial/ multidrug resistance/ pulsed-field gel electrophoresis/ intensive care unit/ healthcare-associated infection分类
医药卫生引用本文复制引用
毛璞,傅威,杨淳,邱桂霞,叶丹,黎毅敏..ICU分离的多重耐药鲍曼不动杆菌PFGE分型及Ⅰ型整合子介导的耐药研究[J].中国感染控制杂志,2012,11(6):417-421,5.基金项目
广州市教育局创新团队科研基金(B94117)广州市科技计划项目(2010J-E171) （B94117）
