中国骨伤2013，Vol.26Issue(2)：142-146,5.DOI:10.3969/j.issn.1003-0034.2013.02.015
牵张应力环境下六味地黄汤含药血清对成骨细胞增殖分化影响的实验研究
Influence of serum containing Liuwei Dihuang decoction（六味地黄汤）on proliferation of osteoblasts under stretchstress environment
摘要
Abstract
Objective:To study influence of serum containing Liuwei Dihuang decoction (六味地黄汤)on proliferation and differentiation of osteoblast form neonatal SD rats cultured in vitro at different times and different stretch stress. Methods: After osteoblast cultured for 24 hours in the serum containing Liuwei Dihuang decoction ( 六味地黄汤 )and serum in control group,the 0.5 Hz frequency ,6% and 12% stretch-stress were added. The MTT and the activity of ALP were measured at the 12th and 24th hours,and the data were analyzed. Results: ①In the environment of stretch stress to the frequency of 0.5 Hz, and stretched for 24 hours, the osteoblast was stimulated under elongation rate of 6% and 12%; the proliferation and differentiation of osteoblast was more active under elongation rate of 12% than that of 6%. ②There were no stimulating effects on osteoblast proliferation and differentiation of serum containing Liuwei Dihuang decoction (六味地黄汤) acted on osteoblast cells of SD rats cultured in vitro for a shot time. Conclusion: Stretch stress environment can enhance osteoblast proliferation and differentiation cultured in vitro.关键词
应力,物理/成骨细胞/细胞增殖/补肾药Key words
Stress, mechanical/Osteoblasts/Cell proliferation/Kidney-reinforcing drugs引用本文复制引用
程婉,汤小康,应航,李敏..牵张应力环境下六味地黄汤含药血清对成骨细胞增殖分化影响的实验研究[J].中国骨伤,2013,26(2):142-146,5.基金项目
浙江省中医药科学研究基金计划(编号:2010ZA020) （编号:2010ZA020）
浙江省自然科学基金(编号:Y2110928) （编号:Y2110928）
