中国科学院研究生院学报2013，Vol.30Issue(1)：18-23,6.DOI:10.7523/j.issn.1002-1175.2013.01.004
通过对免疫共沉淀技术的优化验证蛋白质弱相互作用
Confirmation of weak protein-protein interactions by optimizing co-immunoprecipitation
摘要
Abstract
Co-immunoprecipitation is widely used to detect protein-protein interaction in physiological condition. The single-factor method was used to optimize some factors of co-immunoprecipitation, including component concentrations of cell-lysis buffer, antibody dosage, hCLP46 protein quantity, and cross-linking DSP. As a result, the weak interaction between hCLP46 (human CAP10-like protein 46) and ER chaperone calnexin is confirmed. The present work provides an important basis for further study of the protein-protein interaction.关键词
免疫共沉淀/hCLP46/calnexin/影响因素/蛋白质相互作用Key words
co-immunoprecipitation ( Co-IP)/ hCLP46/ calnexin/ optimization factors/ protein-protein interaction分类
生物科学引用本文复制引用
冯晓琴,徐峰,王嵬,刘利新..通过对免疫共沉淀技术的优化验证蛋白质弱相互作用[J].中国科学院研究生院学报,2013,30(1):18-23,6.基金项目
国家自然科学基金(30670889,30771193)资助 （30670889,30771193）
