中国临床药理学杂志2013，Vol.29Issue(1)：59-61,67,4.
盐酸益母草碱对药流后大鼠内皮素介导的信号通路的影响
Effect of leonurine hydrochloride on ET-mediated signal pathway in incomplete abortion in rats
摘要
Abstract
Objective To explore the mechanism of leonurine hydro-chloride (LH) on uterine contractions in abortion rats. Methods Early pregnancies in rats had incomplete abortions induced using mifepristone in combination with misoprostol. After the abortions, the rats were treated with LH orally for 7 days and surgery was performed. The sinistro -uterus was dissected for measurement of endothelin( ET) and nitric oxide (NO). The dextro - uterus was stored at - 80 ℃ for ETA and ETB analysis. Myometrial cells from the dextro - uterus were cultured for measurement of phospholipase C ( PLC ) activity, intra - cellular Ca2+ concentration ( [ Ca2 + ] i) , and protein kinase C (PKC) activity. Results In vivo experiments, the ET level and ET/NO ratio were increased after LH treatment in rats with induced abortions and LH - can up - regulate ETA mRNA expression (P < 0. 01 , vs the model group) , but there was not ETB mRNA change. LH significantly increased the [ Ca2 + ] i, PLC activity , and relative production of PKC protein in myometrial cells. Conclusion LH increased uterine contractions in rats with incomplete abortions by modulating the ET receptor - mediated signal pathway.关键词
盐酸益母草碱/内皮素/一氧化氮/胞内钙离子浓度Key words
leonurine hydrochloride/ endothelin/ nitric oxide / intra -cellular Ca2+ concentration引用本文复制引用
赵懿清,李霞,袁凤来,贺春晖,吕卫群..盐酸益母草碱对药流后大鼠内皮素介导的信号通路的影响[J].中国临床药理学杂志,2013,29(1):59-61,67,4.基金项目
江苏省中医药管理局中医药科技基金资助项目(LB09094) （LB09094）
青年科学基金资助项目(81101372) （81101372）
