中国临床医学2012，Vol.19Issue(6)：585-587,3.
小鼠心脏移植急性排斥反应模型的建立
Mouse Model of Heart Transplantation with Acute Rejection
摘要
Abstract
Objective:To build a mouse model of heart transplantation for the research of acute transplantation rejection. Methods: The donors and acceptors in the experiment group were BALB/c and C57BL/6 mice respectively(n = 20), and those in the control group were 20 pairs of BALB/c mice. The "cuff" vascular anastomosis technique was used for heterotopic heart transplantation at the neck of mice. The tissues of transplanted hearts from 10 subjects of each group were taken for pathologic observation on Day 7. The survival time of the transplanted hearts were observed in the rest of subjects. Results: The success rate of the operation was 92. 5%. At 7 days after translation, the pathologic examination revealed that the rejection level were 3. 55 ± 0. 44 in the experiment group and 0. 40 ± 0. 32 in the control group, and there was a statistically significant difference between them(F<0. 05). The survival time of the transplanted hearts in the experiment group was(8. 22 ± 0. 67) d, and that in the control group was more than 100 d? and there was a statistically significant difference between them(P<0. 05). Conclusions: A stable mouse model of heart transplantation from BALB/c to C57BL/6 has been built by using the "cuff" method.关键词
心脏移植/小鼠/模型/急性排斥反应Key words
Heart transplantation/ Mouse/ Model/ Acute rejection分类
医药卫生引用本文复制引用
刘琛,王斌,武多娇,王春生..小鼠心脏移植急性排斥反应模型的建立[J].中国临床医学,2012,19(6):585-587,3.基金项目
上海市重点学科建设项目(编号:B116) （编号:B116）
