中国临床医学2012，Vol.19Issue(6)：594-597,4.
5-氮杂胞苷对大鼠骨髓间充质干细胞体外诱导分化的研究
Myogenic Differentiation of Rat Bone Marrow Derived Mesenchymal Stem Cells Induced by 5-Azacytidine in Vitro
摘要
Abstract
Objective:To explore the possibility of inducing myogenic differentiation of rat bone marrow derived mesenchymal stem cells (BMSCs) by 5-azacytidine in vitro. Methods: BMSCs were harvested from 1-week rats, and the surface antigens were identified by flow cytometer. The BMSCs were then treated with 5-azacytidine to achieve the myogenic differentiation. The expression of desmin and crsarcomeric aetin(α-SMA) of the induced BMSCs were detected by immunohistochemical staining. Results: BMSCs from rat long bones could be purified after 3 passages. CD29 and CD105 were positive, while CD34 and CD45 were negative. The immunohistochemical staining revealed the expression of desmin and crSMA in the inducted BMSCs. Conclusions: The myogenic differentiation of BMSCs could be induced by 5-azacytidine.关键词
骨髓间充质干细胞/5-氮杂胞苷/肌样细胞Key words
Bone marrow derived mesenchymal stem cells/5-azacytidine/ Myogenic cells分类
医药卫生引用本文复制引用
孙思,杜小文,徐剑炜..5-氮杂胞苷对大鼠骨髓间充质干细胞体外诱导分化的研究[J].中国临床医学,2012,19(6):594-597,4.基金项目
浙江省科技厅面上项目资助(编号:2008C33022) （编号:2008C33022）
