中国美容医学2013，Vol.22Issue(1)：71-74,4.
葡萄糖对人牙周膜成纤维细胞OPG、RANKL mRNA表达的影响
The effects of high glucose on OPG and RANKL expression in human periodontal ligament fibroblasts
张兰 1孙琳琳 2丁岩 3宋健玲2
作者信息
- 1. 西安交通大学口腔医院牙周黏膜科 陕西西安710004
- 2. 宝鸡市口腔医院
- 3. 无锡市精神卫生中心口腔科
- 折叠
摘要
Abstract
Objective To observe the effects of different concentrations of glucose on mRNA expression of osteoprotegerin (OPG) and receptor activator of NF-kappa B ligand (RANKL) in human periodontal ligament fibroblasts (HPDLF) and investigate the effects of glucose on alveolar bone absorption. Methods The primary HPDLF were cultured according to the method of tissue explant in vitro and identified by immunohistochemistry. HPDLF from passages four to eight were used for experiments. HPDLF were exposed respectively to different concentrations of glucose (0, 5.5, 11.1, 16.7,22.2, 33.3mmol/l). The mRNA expression of OPG and RANKL in HPDLF was detected using semiquantitative reverse transcription polymerase chain reaction (RT-PCR) analysis. Results High concentrations glucose down-regulated mRNA levels of OPG and up-regulated the mRNA levels of RANKL dose-dependently. The ratio of RANKL mRNA to OPG mRNA also increased as the glucose concentration rose. Conclusion The high level glucose in periodontal tissue in diabetes mellitus could down -regulate OPG expression and up -regulated RANKL expression, increase the ratio of RANKL to OPG, activate the differentiation of osteoclasts and promote alveolar bone absorption, which might increase the susceptibility of diabetics to periodontal disease.
关键词
人牙周膜成纤维细胞/葡萄糖/骨保护素/核因子κB受体活化剂配体Key words
Human periodontal ligament fibroblasts/ glucose/ OPG / RANKL分类
生物科学引用本文复制引用
张兰,孙琳琳,丁岩,宋健玲..葡萄糖对人牙周膜成纤维细胞OPG、RANKL mRNA表达的影响[J].中国美容医学,2013,22(1):71-74,4.
