中国脑血管病杂志2012，Vol.9Issue(6)：311-316,6.DOI:10.3969/j.issn.1672-5921.2012.06.007
氦-氖激光对大鼠骨髓间充质干细胞生长及神经分化潜能的作用
Effects of He-Ne laser irradiation on bone marrow mesenchymal stem cell growth and neural differentiation potential
摘要
Abstract
Objective To observe the effects of He-Ne laser therapy instrument on bone marrow mesenchymal stem cell ( BMSC) growth and neural differentiation potential. Methods The primary BMSCs from Sprague-Dawley rats were Laken using whole bone marrow culture. The passage 3 cells were randomly divided into an experimental group ( He-Ne laser therapy instrument Irealed for 10 min) and a conlrol group ( exposing in the air for the same time) according to whether they were Ireated wilh the He-Ne laser therapy inslrumenl or nol. The cell proliferation aclivily was measured wilh cell counting kit-8 ( CCK-8 ) assay. Apoptosis detection was performed using flow cytometry. The cell vitality was detected by Trypan blue staining. Human neural stem cell differentiation potential was identified by immunofluorescence tech-nique. Results ①Cells showed a fibroblast-like adherent growth. The phenotypic identification results revealed thai the expressions of CD29 and CD90 were positive, but the expression of CD45 was negative, and it is consistent with the characteristics of the BMSC phenotype. ②There was no significant difference in the proliferative activity between the experiment group and the controt group,P ＞ 0. 05. ③There were no significant differences between the early apoptosis rate (1. 10 ± 0. 26% ) in the experiment group and that (1.43±0.06%) in the control group, as well as the cell viability (96. 2 ± 0. 6% ) in experiment group and that (96.2 ±0.7%) in the control group. ④Similar io the control group, BMSCs in the experiment group showed the suspended neurospheres after being cultured in neural stem cell culture medium. Immu-nofluorescence staining showed that the nestin slaining of neurosphere cetts was positive. Conclusion He-Ne laser therapy instrument has no significant effects on the proliferation and viability of BMSCs. It does not cause apoptosis and change the potential of BMSCs to neural stem cell differentiation.关键词
激光/骨髓间充质干细胞/增殖/分化/神经干细胞/大鼠/体外研究Key words
Laser/ Bone marrow stromal stem cells/ Proliferation/ Differentiation/ Neural stem cells/ Rats/ In vilro引用本文复制引用
毕迎立,范建中,陈银海..氦-氖激光对大鼠骨髓间充质干细胞生长及神经分化潜能的作用[J].中国脑血管病杂志,2012,9(6):311-316,6.基金项目
广东省自然科学基金项目(8451051501000460) （8451051501000460）
广州市海珠区科技计划项目(2009-Y-018) （2009-Y-018）
