中国农业大学学报2013，Vol.18Issue(1)：88-93,6.
珠眉海棠(Malus zumi Mats)盐应答MzSTO基因的克隆与表达分析
Cloning and expression analysis of salt stress MzSTO gene from Malus zumi Mats
摘要
Abstract
The full-length cDNA sequence of MzSTO gene was isolated from a cDNA library of Malus zumi. The full-length cDNA of MzSTO was 1 066 bp. The 5'-UTR and the 3'-UTR were 49 and 288 bp respectively. Its open reading frame of 729 bp encoded a protein of 242 amino acids. There were two B-box zinc-finger domains (CX2CX8CX7CX2CX4HX8H) near N-terminal. MzSTO was induced by low temperature stress and repressed by high-salinity and drought treatment, which was, also responsive to ABA (abscisic acid) treatment by semi-quantative RT-PCR. All the results showed that MzSTO was involved in response of abiotic stresses including salt, drought and cold stresses through a possible ABA-dependent pathway in Malus zumi.关键词
珠眉海棠/MzSTO/非生物胁迫/RT-PCRKey words
Malus zumi Mats/ MzSTO/ abiotic stresses/ RT-PCR分类
农业科技引用本文复制引用
蒋玉妆,李凌子,赵宇,李擎天,梁占锋,孔瑾..珠眉海棠(Malus zumi Mats)盐应答MzSTO基因的克隆与表达分析[J].中国农业大学学报,2013,18(1):88-93,6.基金项目
国家自然科学基金资助项目(31171940) （31171940）
北京市自然科学基金资助项目(6112012) （6112012）
