中国农业大学学报2013，Vol.18Issue(1)：128-133,6.
秋水仙素诱导细叶百合多倍体研究
Colchicines-induced polyploid plants and identification in Lilium pumilum DC
摘要
Abstract
This work was aimed to obtain tetraploid Lilium pumilum DC with excellent ornamental traits and agronomic characters,and provide better cross parent for breeding of new Lilium variety. The seeds of Lilium pumilum DC (2n = 2x=24) were treated with different concentrations of colchicine solution at different hours in order to figure out a proper method and obtain polyploids. The results indicated that the most effective induction was obtained by 0.1 % colchicine treatment for 24 hours with 30 % mutation rate. By chromosome observation of root tip, the tetraploid plants were successfully obtained with the chromosome number In =4x = 48. The tetraploid plants showed obvious characteristics of low stoma density,large stoma,slow growth,thick and wide leaves and dark leaf color at the initial stage,but quick growth and large leaves at later stage. The results indicated that the polyploid plants were identified by phenotype, stomas and chromosome number observation.关键词
细叶百合/秋水仙素/染色体/多倍体Key words
Lilium pumilum DC/ colchicine/ chromosome/ polyploid分类
农业科技引用本文复制引用
杨英杰,葛蓓孛,魏倩,高俊平,洪波..秋水仙素诱导细叶百合多倍体研究[J].中国农业大学学报,2013,18(1):128-133,6.基金项目
国家公益性行业(农业)科研专项经费(200903020) （农业）
