中国农业大学学报2013，Vol.18Issue(1)：134-141,8.
热应激对不同品种鸡繁殖性能、血液生殖激素及相关基因mRNA表达量的影响
Effects of heat stress on reproductive performance,blood reproductive hormone concentrations and gene mRNA expression in different varieties of chicken
摘要
Abstract
This study is to explore the effects of thermal stress on reproductive performance, blood reproductive hormones and genes mRNA expression to the country local variety and foreign cultivate layers varieties. Test choice body condition similar 16 Ninety-six sixteen-week Jining Bairichicken and 96 Hy-line variety Brown chicken were random grouped into optimum temperature group (25± 0.26) ℃ and High temperature group (38 ± 0.26)℃ testing for 28 days. Each group has eight repeats with 6 chicken in each repeat. Ovary weight, follicle total and blood reproductive hormone concentration and reproductive shaft related gene expression mRNA relative quantity were determination at 112,119,126,and 140 days of age. The results show that: 1) Ovarian and total follicular of the Hailan brown chicken developed better than which in Jining Bairi chicken under the optimum temperature conditions, however, Jining Bairi chicken and Hy-line variety Brown chicken appeared obvious ovarian development hindered, total follicle number hyperplasia slow (P<0.05) at 7th days of the heat stress. 2) in the whole thermal stress period,high temperature group of follicle stimulating hormone (FSH) and luteinizing hormone (LH) hormone content significant change (P< 0. 05),heat stress after 7d estradiol (E2) was significantly reduced (P<0.05) ,progesterone (P4) increased at first, then dropped significantly (P<0. 05);CRH level significantly higher than that of optimum temperature group (P< 0. 05),reed jining one hundred days chicken high temperature group of each blood reproductive hormone level of the coefficient of variation of the Hy-line variety Brown,and no significant difference (P>0.05).3) optimum temperature treatment group of reed jining one hundred days chicken GnRh,PRL,FSH-β,ESR,LHR,FSHR mRNA expression quantity are Hy-line variety Brown chicken,but no significant difference(P>0.05) ;and high temperature group reed Jining Bairi chicken mRNA expression quantity except outside PRL significantly Hy-line variety Brown chicken (P<0. 05). The results indicate,local variety in thermal stress condition ovarian,total foilicular development and the blood reproductive shaft relevant hormone secretion regulation function better than foreign varieties; And reproductive shaft relevant hormone mRNA expression quantity changes with various effect.关键词
鸡/热应激/生殖激素/mRNA表达量/卵巢Key words
chickens thermal stress/ reproductive hormone/ mRNA expression quantity/ ovarian分类
农业科技引用本文复制引用
李永洙,CUI Yong-quan..热应激对不同品种鸡繁殖性能、血液生殖激素及相关基因mRNA表达量的影响[J].中国农业大学学报,2013,18(1):134-141,8.基金项目
山东省临沂市科技攻关项目(201012009) （201012009）
临沂大学博士科研启动基金(BS07011) （BS07011）
日本学生支援机构归国外国留学生短期研究制度基金项目(JP22GR037) （JP22GR037）
