中国农业科学2012，Vol.45Issue(22)：4651-4659,9.DOI:10.3864/j.issn.0578-1752.2012.22.011
草莓十二倍体种间杂种的获得及其回交研究
Obtaining Dodecaploid Interspecific Hybrid in Strawberry and Its Backcross
摘要
Abstract
[Objective] The backcross between higher ploid interspecific hybrids and strawberry cultivars was beneficial to broadening genetic background of modern cultivated strawberry and breeding of new cultivars. [Method] The dodecploid interspecific hybrid YH15-10 (2n=12x=84) was obtained from the cross between Fragaria x ananassa cv. Yuhime (2n=8x=56) and the wild pentaploid strawberry accession Heilongjiang 7 (2n=5x=35) native to China. The backcross between the dodecaploid interspecific hybrid YH15-10 and strawberry cultivars 'Hokowase' and 'Honeoye' were carried out. The ploidy, botanical characteristics, pollen size and viability, and fruit traits of the offspring were investigated. [Result] The YH15-10 had relatively few leaves, 4.4 inflorescences per plant and 8 to 25 flowers per inflorescence in the open field. The fruits were red, mostly abnormal, in which the maximum fruit weight was 10.5 g. The flesh was yellowish white with obvious aroma. When YH15-10 was used as female parent, no seedlings were obtained. When YH15-10 was used as male parent, 83 offspring was obtained. The dodecploid YH15-10 had large, middle and small pollens, and the pollen viability was similar to that of strawberry cultivars. The octoploid, decaploid, hendecaploid and dodecploid seedlings were obtained from Hokowase × YH15-10. Two seedlings 1H1-5 (l0x) and IH1-7 (8x) with high vitamin C of 0.95 mg·g-1 and 1.07 mg·g-1, respectively, were selected. Their fruits were sweet, aromatic, but small, with the maximum fruit weight of 10.4 g and 9.6 g, respectively. [ Conclusion] This paper gave a good example for obtaining higher ploid strawberry. The dodecploid interspecific hybrid YH15-10 was helpful to develop new strawberry cultivars. Some seedlings with excellent quality, high vitamin C and high aromatic compound content were obtained from the backcross between the dodecaploid interspecific hybrid YH15-10 and strawberry cultivars. These high ploidy offspring could be further used as the parents to breed new strawberry cultivars.关键词
草莓/种间杂交/十二倍体/回交Key words
strawberry (Fragaria spp.)/ interspecific hybridization/ dodecaploid/ backcross引用本文复制引用
雷家军,薛莉,代汉萍..草莓十二倍体种间杂种的获得及其回交研究[J].中国农业科学,2012,45(22):4651-4659,9.基金项目
国家自然科学基金(30971976) （30971976）
