中国农业科学2012，Vol.45Issue(22)：4678-4687,10.DOI:10.3864/j.issn.0578-1752.2012.22.014
湖羊GHR和IGF-Ⅰ基因表达的发育性变化及其与肉质性状的关联
Developmental Changes of Gene Expression of GHR and IGF-Ⅰ Genes and Their Association Analysis with Meat Quality Traits in Hu Sheep
摘要
Abstract
[Objective] The objective of this study was to discuss the developmental changes of gene expression of growth hormone receptor (GHR) and insulin-like growth factor- I (IGF-1) and their association analysis with meat quality traits , and the relationship of the expression level between GHR gene and IGF-1 gene in Hu sheep. [Method] The developmental changes of gene expression of GHR gene and IGF- I gene on the early growth of Hu sheep were detected by real-time fluorescent quantitative RT-PCR. [Result] The results were as followes: With increasing age after birth, the GHR expression trend of ewes was up-down-up-down-up, and 6-month reached the top. The GHR expression trend of rams was that 2-day-old to 2-month-old was increasing, 2-month-old to 4-month-old was decreasing, 4-month-old to 6-month-old was increasing and reached the top. With the increasing age after birth, the IGF- I gene expression trend of ewes was increasing from 2-day-old to 6-month-old which had the top expression. The IGF- I gene expression trend of rams was that at 2-day-old firstly increased to a peak at one-month-old, 1-month-old to 3-month-old had a decreasing process and reached the same level which was close to the newborn, and then increased with the increasing age, and 6-month-old reached the top. Most growth phases had a significant difference or extreme significant difference in GHR and IGF-1 and the expressions of GHR and IGF-1 between most rams and ewes that at the same growth phase had a significant difference or extreme significant difference. The results showed that the expressions of GHR and IGF- I had a significant positive correlation, (0.01 <P<0.05) the expressions of GHR and IGF- I genes had a extreme significant positive correlation (P< 0.01) with diameter of muscle fibers and a extreme significant negative correlation (P<0.01) with density of muscle fibers, and the expressions of GHR had a extreme significant positive correlation (P<0.01) with shear force of muscle fibers, the expression of IGF-1 had a significant positive correlation with shear force of muscle fibers (0.01<P<0.05). [Conclusion] The results showed that different stages and different genders had important influences on the expressions of GHR and IGF-1 genes in sheep skeletal muscle. After birth, the expressions of the two genes did not always increase or decrease, the turning points of different genes expression were not at the same time. The expression of GHR and IGF- I had a significant positive correlation in early muscle traits of Hu sheep. GHR and IGF- I gene can be regarded as the candidate gene for the trait of meat quality in Hu Sheep.关键词
湖羊/GHR基因/IGF-Ⅰ基因/基因表达/肉质性状Key words
Hu sheep/ GHR gene/ IGF-1 gene/ gene expression/ meat quality traits引用本文复制引用
孙伟,吴文忠,周洪,李达,马月辉,关伟军,储明星,丁家桐,李碧春,张有法,陈玲..湖羊GHR和IGF-Ⅰ基因表达的发育性变化及其与肉质性状的关联[J].中国农业科学,2012,45(22):4678-4687,10.基金项目
中国博士后科学基金特别资助项目(200902154)、科技部家养动物种质资源平台、现代肉羊产业技术体系建设(CARS-39)、江苏高校优势学科建设工程资助项目、江苏省农业科技支撑计划项目(BE2012331)、江苏省六大人才高峰项目、国家"863"计划项目(2011AA100307-04)、江苏省苏北科技发展计划(BN2010004)、苏州市农业科技支撑计划(SNG0911)、徐州市农业科技支撑计划(XF11C016) （200902154）
