中国农业科学2012，Vol.45Issue(22)：4697-4704,8.DOI:10.3864/j.issn.0578-1752.2012.22.016
东方蜜蜂微孢子虫对密林熊蜂的致病机理
Pathogenic Mechanism of Bombus patagiatus Infected by Nosema ceranae
摘要
Abstract
[Objective] The objective of this study is to define the infectivity and pathogenic mechanism of Nosema ceranae to Bombus patagiatus. [Method] Traditional biology and ultrastructure under electronic microscope methods were used, and combined with the quantitative real-time PCR (qPCR) to explore the pathogenesis of N. ceranae to B. patagiatus. [Result] In the initial infection, the infected bees did not exhibit obvious external disease signs except decreased feeding and motor retardation. In the late period, the infected bees exhibited dispirited, weak and unable to fly. Through observation with light microscope, few N. ceranae but a lot of bacteria were found in the midgut of bees. N. ceranae mainly infected midgut of epithelial cells, the nuclear enlarged and out of shape, mitochondria become smaller even disintegrating and endoplasmic reticulum become disorders. However, the spores only infected the cytoplasm of the host instead of invading the nuclear, which lead to the disintegration of the mitochondria and cytoclasis. Quantitative analysis on RT-PCR showed that N. ceranae reached the highest level in the midgut and fat body 3-4 d after being infected and other organizations were barely detected. [ Conclusion] N. ceranae can cross-species infect B. patagiatus and the pathological process starts with the pathological changes of intestinal cells, which lead to the cytoclasis and apoptosis.关键词
密林熊蜂/东方蜜蜂微孢子虫/超微结构/qPCRKey words
Bombus patagiatus/ Nosema ceranae/ ultrastructure/ qPCR引用本文复制引用
秦浩然,和绍禹,吴杰,李继莲..东方蜜蜂微孢子虫对密林熊蜂的致病机理[J].中国农业科学,2012,45(22):4697-4704,8.基金项目
国际科技合作项目(2009DFA32600)、国家自然科学基金(30972149,30700606)、北京市自然基金(6122032)、国家蜂产业技术体系建设专项(CARS-45)、国家公益性行业(农业)科研专项(201203080-4) （2009DFA32600）
