中国农业科学2012，Vol.45Issue(22)：4720-4727,8.DOI:10.3864/j.issn.0578-1752.2012.22.019
稻曲毒素A的相对含量分析及其与致病力的相关性
Detection of the Relative Content of Ustiloxin A in Rice False Smut Balls and Correlation Analysis Between Pathogenicity and Ustiloxin A Production of Ustilaginoidea virens
摘要
Abstract
[Objective] The objective of this study is to define the active components of crude toxin produced by Ustilaginoidea virens, to analyze the ustiloxin A production of false smut balls in different areas, and to discover the correlation between pathogenicity and ustiloxin A production of the fungus. [ Method] The crude toxin of U. virens was extracted from false smut balls by acetone, methanol and water, respectively, and the toxic effect was assayed and indicated by inhibition to wheat seed germination. The active components of the crude toxins were studied by liquid chromatography mass-spectrometry (LC-MS) and UV absorption spectra. Ustiloxin A production of 224 false smut balls collected from different areas in China was analyzed by high performance liquid chromatography (HPLC). The pathogenicity of 52 isolates of U. virens was examined through inoculating the susceptible rice cultivars, Liangyoupeijiu. The correlation between pathogenicity and ustiloxin A production of the fungus was analyzed by using SPSS software. [Result] The crude toxin extracted with water and methanol had higher inhibition to wheat radicle growth with the inhibition rate of 59.6% and 52.9%, respectively, while the crude toxin extracted with acetone had lower inhibition. The molecular weight of the active ingredient II was 673.9 by determination with LC-MS. Combined with the UV spectra assay, ingredient II could be proved to be ustiloxin A. It was found that there was a significant difference in the ustiloxin A production among false smut balls collected from different areas. However, correlation analysis indicated that no significant correlation between pathogenicity of the fungus and its ustiloxin A production (R=0.222, P=0.114 ) . [Conclusion] Water extract of false smut balls caused by U. virens contained ustiloxin A. And a significant difference in ustiloxin A production was found in false smut balls collected from different areas. There may be no association between pathogenicity and ustiloxin A production of the fungus.关键词
稻曲菌/粗毒素/稻曲毒素A/致病力Key words
Ustilaginoidea virens/ crude toxin/ ustiloxin A/ pathogenicity引用本文复制引用
尹小乐,陈志谊,刘永锋,于俊杰,李燕,俞咪娜..稻曲毒素A的相对含量分析及其与致病力的相关性[J].中国农业科学,2012,45(22):4720-4727,8.基金项目
国家公益性行业(农业)科研专项(200903039-2)、江苏自主创新特色产业技术体系(CX(12)1003) （农业）
