中国普通外科杂志2013，Vol.22Issue(1)：59-62,4.DOI:10.7659/j.issn.1005-6947.2013.01.014
GPC-3mRNA与GGT联合检测诊断AFP阴性原发性肝细胞肝癌
Combination detection of GPC-3 mRNA and GGT for diagnosis of AFP-negative hepatocellular carcinoma
摘要
Abstract
Objective: To investigate the diagnostic value of the combination detection of the glypican 3 (GPC-3) mRNA expression in peripheral blood monouclear cells (PBMC) and plasma level of γ-glutamyl transpeptidase (GGT) for patients with a-fetoprotein (AFP)-negative hepatocellular carcinoma (HCC).Methods: The PBMC GPC-3 mRNA expressions in 39 patients with AFP-negative HCC, 39 patients with non-HCC liver diseases and 40 healthy volunteers were determined by RT-PCR method, and the plasma GGT levels (>58 U/L was considered as positive) in the 39 AFP-negative HCC cases were measured by using automatic biochemical analyzer. The positive rates by single or combination of the two detections were analyzed. Results: The detection rate of PBMC GPC-3 mRNA expression in the 39 AFP-negative HCC patients was 62% (24/39), while none of the cases in patients with non-HCC liver diseases and healthy subjects presented positive expression of PBMC GPC-3 mRNA, thus the specificity of PBMC GPC-3 mRNA for AFP-negative HCC was 100%. The positive rate of GGT elevation in the 39 AFP-negative HCC patients was 80% (31/39). The positive rate of the combination detection of PBMC GPC-3 mRNA and GGT reached 95%, which was significantly higher than that of either GPC-3 mRNA or GGT alone (both P<0.05).Conclusion: PBMC GPC-3 mRNA expression has a high specificity in AFP-negative HCC patients. The combination detection of GPC-3 mRNA and GGT is helpful to the early diagnosis of AFP-negative HCC.关键词
癌,肝细胞/诊断/磷脂酰肌醇蛋白聚糖类/γ-谷氨酰转移酶Key words
Carcinoma, Hepatocellular/diag/ Glypicans/ y-Glutamyltransferase分类
医药卫生引用本文复制引用
范钦桥,陈书伟,陈小雪,杨正,袁超,黄军..GPC-3mRNA与GGT联合检测诊断AFP阴性原发性肝细胞肝癌[J].中国普通外科杂志,2013,22(1):59-62,4.基金项目
湘南学院立项课题(09Y033). （09Y033）
