中国蔬菜Issue(2)：27-34,8.
48份分蘖洋葱种质资源遗传多样性的ISSR标记和农艺性状分析
ISSR Markers of 48 Tillering Onion (Allium cepa var.aggregatum) Germplasms Genetic Diversity and Analysis of Their Agronomic Traits
摘要
Abstract
Inter-simple sequence repeat ( ISSR ) markers and agronomic traits were analyzed to observe the genetic diversity of 48 tillering onion ( Allium cepa var. aggregatum ) germplasms from Heilongjiang and Jilin Provinces in China. Thirteen ISSR primers with rich polymorphism, stable amplification and good repeatability were screened from 51 ISSR primers. With these screened primers, 132 bands were identified, of which 109 bands were polymorphic. The genetic similarity was estimated with Nei-Li coefficient, and the results showed that the genetic similarity coefficients of 48 tillering onion germplasms ranged from 0.59 to 0.94. The investigation was also carried out on their agronomic traits, including bolting, leaf color, seed coat color, and single fruit quality. The results showed that the genetic similarity coefficients of these 48 tillering onion germplasms ranged from 0.38 to 1.00. Both results indicated that the genetic diversity of 48 tillering onion germplasms was rich. The clustering results of these 2 methods were basically identical, and the clustering results had obvious connection with their geographic origins.关键词
分蘖洋葱/ISSR标记/农艺性状/遗传多样性Key words
Tillering onion/ISSR marker/Agronomic traits/Genetic diversity分类
农业科技引用本文复制引用
金雪,周新刚,刘守伟,刘淑芹,吴凤芝..48份分蘖洋葱种质资源遗传多样性的ISSR标记和农艺性状分析[J].中国蔬菜,2013,(2):27-34,8.基金项目
国家自然科学基金项目(30971998,31172002),国家"973"项目专题(2009CB119004-05),大宗蔬菜产业技术体系项目(CARS-25-08) （30971998,31172002）
