中国蔬菜Issue(4)：38-45,8.
杨凌番茄黄化曲叶病毒外壳蛋白基因的克隆与其核心序列分析
Clone and Core Sequence Analysis on CP Gene of TYLCV Isolate from Tomato in Yangling
摘要
Abstract
Taking the tomato plants with TYLCV symptom obtained in Yangling tomato production base and named TYLCV-Y1 ( GenBank accession; KC293824) as material, we cloned the full coat protein gene ( CP ) and the core part (tCP ) of coat protein gene of the TYLCV-Y1 isolate and analyzed the sequence features and tCP evolutionary character. Multiple comparison of this virus full coat protein sequence with other 18 TYLCV-CP nucleotide sequence and constructed evolutionary tree based on CP gene nucleotide sequence using DNAMAN software. The results indicated that the tomato yellow leaf curl virus isolation ( TYLCV-Y1) obtained in Yangling was confirmed as the resource of TYLCV-Y1. We also acquired the full gene sequence of coat protein of TYLCV-Y1, which lay foundation for resistance based on CP gene. The core sequence tCP is very conservative and its length is about 419 bp. This core sequence coded 137 amino acids and has a trans-membrane structure from 79 to 97 amino acid. Based on phylogenetic tree constructed by CP gene, TYLCV from NCBI was divided into 3 subgroups: TYLCV subgroup Ⅰ ,TYLCV subgroup Ⅱ and TYLCV subgroup Ⅲ. The isolate TYLCV-Y1 was belonged to subgroup Ⅰ .关键词
番茄黄化曲叶病毒/杨凌番茄黄化曲叶病毒分离物/克隆/序列分析Key words
Tomato yellow leafcurl virus (TYLCV )/ TYLCV isolation from Yangling/ Cloning/ Sequence analysis分类
农业科技引用本文复制引用
李云洲,梁燕,王巧丽,李翠,崔霞,秦蕾..杨凌番茄黄化曲叶病毒外壳蛋白基因的克隆与其核心序列分析[J].中国蔬菜,2013,(4):38-45,8.基金项目
陕西省科技统筹创新工程计划项目(2011KTCL02-03),陕西省"13115"科技创新工程重大科技专项(2009ZDKG-14) （2011KTCL02-03）
