中国水稻科学2013，Vol.27Issue(1)：41-48,8.DOI:10.3969/j.issn.1001-7216.2013.01.006
基于SSR标记的云南地方稻种群体内遗传多样性分析
Analysis of Genetic Diversity within Populations of Rice Landraces from Yunnan Using Microsatellite Markers
摘要
Abstract
Genetic diversity within populations of 16 rice landraces and 2 improved cultivars from Yunnan Province were analyzed using 20 SSR markers. The results showed that the SSR marker polymorphism within populations for 87. 5% of rice landraces was higher than that of the improved cultivars, while the polymorphism within populations for 12. 5% of landraces was similar with that of the improved cultivars. For 81. 2% of rice landraces, the number of alleles (Na) and Nei's genetic diversity index (He), were higher than those of the improved cultivars, and for 18. 8% of landraces, the figures were same as or slightly smaller than those of the improved cultivars. The He within populations of rice landraces significantly differed, ranging from 0. 0146 to 0. 5117, while Huangbansuo-1 ( Huangbansuo collected in 1980), Huangbansuo-2(Huangbansuo collected in 2007), Maxiangu-1(Maxiangu collected in 1980) and Maxiangu-2 (Maxiangu collected in 2007) were the highest with figures of 0. 2327, 0. 4214, 0. 5117 and 0. 4489, respectively. Heterozygosity within rice varieties for 87. 5% of rice landraces was significantly higher than that of the improved cultivars. AMOVA showed that 1/4 genetic difference among rice landraces were from within populations of rice landraces(P<0. 001). The polymorphism, Ne, He and percentage of variation within populations of rice landraces at RM333, RM257 and RM180 were higher than others. It was speculated that RM333, RM257 and RM180 were suitable for testing genetic diversity within rice landraces of Yunnan Province.关键词
水稻/地方品种/群体内遗传多样性/微卫星标记Key words
rice/ landrace/ genetic diversity within population/ microsatellite marker分类
生物科学引用本文复制引用
孙建昌,余滕琼,汤翠凤,曹桂兰,徐福荣,韩龙植..基于SSR标记的云南地方稻种群体内遗传多样性分析[J].中国水稻科学,2013,27(1):41-48,8.基金项目
国家科技支撑计划资助项目(2006BAD13B01) （2006BAD13B01）
农业部作物种质资源保护项目(NB2010-2130135-25-01). （NB2010-2130135-25-01）
