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Use of FK506 and bone marrow mesenchymal stem cells for rat hind limb allografts

Youxin Song Zhujun Wang Zhixue Wang Hong Zhang Xiaohui Li Bin Chen

中国神经再生研究（英文版）2012，Vol.7Issue(34)：2681-2688,8.

中国神经再生研究（英文版）2012，Vol.7Issue(34)：2681-2688,8.DOI:10.3969/j.issn.1673-5374.2012.34.005

Use of FK506 and bone marrow mesenchymal stem cells for rat hind limb allografts

Youxin Song ¹Zhujun Wang ²Zhixue Wang ³Hong Zhang ⁴Xiaohui Li ²Bin Chen¹

作者信息

1. Department of Orthopedics, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China
2. Department of Research, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China
3. Department of Anesthesia, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China
4. Department of Surgery, Affiliated Hospital of Chengde Medical College, Chengde 067000, Hebei Province, China
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摘要

Abstract

Dark Agouti rat donor hind limbs were orthotopically transplanted into Lewis rat recipients to verify the effects of bone marrow mesenchymal stem cells on neural regeneration and functional recovery of allotransplanted limbs in the microenvironment of immunotolerance. bone marrow mesenchymal stem cells were intramuscularly (gluteus maximus) injected with FK506 (tacrolimus) daily, and were transplanted to the injured nerves. Results indicated that the allograft group not receiving therapy showed severe rejection, with transplanted limbs detaching at 10 days after transplantation with complete necrosis. The number of myelinated axons and Schwann cells in the FK506 and FK506 + bone marrow mesenchymal stem cells groups were significantly increased. We observed a lesser degree of gastrocnemius muscle degeneration, and increased polymorphic fibers along with other pathological changes in the FK506 + bone marrow mesenchymal stem cells group. The FK506 + bone marrow mesenchymal stem cells group showed significantly better recovery than the autograft and FK506 groups. The results demonstrated that FK506 improved the immune microenvironment. FK506 combined with bone marrow mesenchymal stem cells significantly promoted sciatic nerve regeneration, and improved sensory recovery and motor function in hind limb allotransplant.

关键词

FK506 (tacrolimus)/bone marrow mesenchymal stem cells/allotransplant/hind limb transplant/function recovery/sensory function/motor function/peripheral nerve injury/regeneration/neural regeneration

Key words

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Youxin Song,Zhujun Wang,Zhixue Wang,Hong Zhang,Xiaohui Li,Bin Chen..Use of FK506 and bone marrow mesenchymal stem cells for rat hind limb allografts [J].中国神经再生研究（英文版）,2012,7(34):2681-2688,8.

基金项目

This work was supported by the National Natural Science Foundation of China,No.30801171 （）

the Natural Science Foundation of Hebei Province,No.C2009001013 and No.H2012406015. （）

中国神经再生研究（英文版）

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ISSN：1673-5374

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