首页|期刊导航|中国神经再生研究（英文版）|Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium
中国神经再生研究（英文版）2012，Vol.7Issue(36)：2907-2914,8.DOI:10.3969/j.issn.1673-5374-2012.36.008
Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium
Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium
摘要
Abstract
The present study co-cultured human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells in complete culture medium- containing cerebrospinal fluid. Enzyme linked immunosorbent assay was used to detect nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor secretion in the supernatant of co-cultured cells. Results showed that the number of all cell types reached a peak at 7–10 days, and the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor peaked at 9 days. Levels of secreted nerve growth factor were four-fold higher than brain-derived neurotrophic factor, which was three-fold higher than glial cell line-derived neurotrophic factor. Increasing concentrations of cerebrospinal fluid (10%, 20% and 30%) in the growth medium caused a decrease of neurotrophic factor secretion. Results indicated co-culture of human embryonic olfactory ensheathing cells, human Schwann cells, human amniotic epithelial cells and human vascular endothelial cells improved the expression of nerve growth factor, brain-derived neurotrophic factor, and glial cell line-derived neurotrophic factor. The reduction of cerebrospinal fluid extravasation at the transplant site after spinal cord injury is beneficial for the survival and secretion of neurotrophic factors from transplanted cells.关键词
olfactory ensheathing cells/Schwann cells/amniotic epithelial cells/vascular endothelial cells/nerve growth factor/brain-derived neurotrophic factor/glial cell line-derived neurotrophic factor/cerebrospinal fluid/regeneration/neural regenerationKey words
olfactory ensheathing cells/Schwann cells/amniotic epithelial cells/vascular endothelial cells/nerve growth factor/brain-derived neurotrophic factor/glial cell line-derived neurotrophic factor/cerebrospinal fluid/regeneration/neural regeneration引用本文复制引用
Sanjiang Feng,Minghua Zhuang,Rui Wu..Secretion of nerve growth factor, brain-derived neurotrophic factor, and glial cell-line derived neurotrophic factor in co-culture of four cell types in cerebrospinal fluid-containing medium[J].中国神经再生研究（英文版）,2012,7(36):2907-2914,8.基金项目
This study was supported by the Science and Technology Development Program of Guangdong Province,No.2009b030801329. （）
