首页|期刊导航|中国神经再生研究（英文版）|Expression of netrin-1 and its receptors, deleted in colorectal cancer and uncoordinated locomotion-5 homolog B, in rat brain following focal cerebral ischemia reperfusion injury
中国神经再生研究（英文版）2013，Vol.8Issue(1)：64-69,6.DOI:10.3969/j.issn.1673-5374.2013.01.008
Expression of netrin-1 and its receptors, deleted in colorectal cancer and uncoordinated locomotion-5 homolog B, in rat brain following focal cerebral ischemia reperfusion injury
Expression of netrin-1 and its receptors, deleted in colorectal cancer and uncoordinated locomotion-5 homolog B, in rat brain following focal cerebral ischemia reperfusion injury
摘要
Abstract
Netrin-1 is currently one of the most highly studied axon guidance factors. Netrin-1 is widely expressed in the embryonic central nervous system, and together with the deleted in colorectal cancer and uncoordinated locomotion-5 homolog B receptors, netrin-1 plays a guiding role in the construction of neural conduction pathways and the directional migration of neuronal cells. In this study, we established a rat middle cerebral artery ischemia reperfusion model using the intraluminal thread technique. Immunofluorescence microscopy showed that the expression of netrin-1 and deleted in colorectal cancer in the ischemic penumbra was upregulated at 1 day after reperfusion, reached a peak at 14 days, and decreased at 21 days. There was no obvious change in the expression of uncoordinated locomotion-5 homolog B during this time period. Double immunofluorescence labeling revealed that netrin-1 was expressed in neuronal cells and around small vessels, but not in astrocytes and microglia, while deleted in colorectal cancer was localized in the cell membranes and protrusions of neurons and astrocytes. Our experimental findings indicate that netrin-1 may be involved in post-ischemic repair and neuronal protection via deleted in colorectal cancer receptors.关键词
neural regeneration/brain injury/cerebral ischemia and reperfusion/netrin-1/uncoordinated locomotion-5 homolog B/deleted in colorectal cancer/neuron/brain injury/grant-supported paper/photographs-containing paper/neuroregenerationKey words
neural regeneration/brain injury/cerebral ischemia and reperfusion/netrin-1/uncoordinated locomotion-5 homolog B/deleted in colorectal cancer/neuron/brain injury/grant-supported paper/photographs-containing paper/neuroregeneration引用本文复制引用
Xiaodan Wang,Jinming Xu,Jieqin Gong,Hui Shen,Xiaoping Wang..Expression of netrin-1 and its receptors, deleted in colorectal cancer and uncoordinated locomotion-5 homolog B, in rat brain following focal cerebral ischemia reperfusion injury[J].中国神经再生研究（英文版）,2013,8(1):64-69,6.基金项目
This study was supported by the Science and Technology Program of Suzhou Industrial Park in China. （）
