中国神经再生研究（英文版）2013，Vol.8Issue(2)：101-110,10.DOI:10.3969/j.issn.1673-5374.2013.02.001
Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats
Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats
Zhengang Sun 1Lingyun Hu 2Yimin Wen 2Keming Chen 3Zhenjuan Sun 2Haiyuan Yue 4Chao Zhang4
作者信息
- 1. Department of Spine Surgery, First People's Hospital of Qingdao Economic & Technological Development Area, Qingdao 266555, Shandong Province, China
- 2. Second Clinical Medical College, Lanzhou University, Lanzhou 730000, Gansu Province, China
- 3. Department of Orthopedics, Nanchong Central Hospital, Nanchong 637000, Sichuan Province, China
- 4. Department of Spine Surgery, Lanzhou General Hospital of Lanzhou Military Region, Lanzhou 730050, Gansu Province, China
- 折叠
摘要
Abstract
The mammalian target of rapamycin (mTOR) pathway plays an important role in neuronal growth, proliferation and differentiation. To better understand the role of mTOR pathway involved in the induction of spinal cord injury, rat models of spinal cord injury were established by modified Allen's stall method and interfered for 7 days by intraperitoneal administration of mTOR activator adenosine the Basso, Beattie and Bresnahan locomotor rating scale was used to evaluate rat locomotor function, and immunohistochemical staining and western blot analysis were used to detect the expression of nestin (neural stem cell marker), neuronal nuclei (neuronal marker), neuron specific enolase, neurofilament protein 200 (axonal marker), glial fibrillary acidic protein (astrocyte marker), Akt, mTOR and signal transduction and activator of transcription 3 (STAT3). Results showed that adenosine triphosphate-mediated Akt/mTOR/STAT3 pathway increased endogenous neural stem cells, induced neurogenesis and axonal growth, inhibited excessive astrogliosis and improved the locomotor function of rats with spinal cord injury.关键词
neural regeneration/spinal cord injury/serine/threonine-specific protein kinase/mammalian target of rapamycin pathway/signal transduction and activator of transcription 3/adenosine triphosphate/signal pathway/rapamycin/photographs-containing paper/ neurorKey words
neural regeneration/spinal cord injury/serine/threonine-specific protein kinase/mammalian target of rapamycin pathway/signal transduction and activator of transcription 3/adenosine triphosphate/signal pathway/rapamycin/photographs-containing paper/ neuror引用本文复制引用
Zhengang Sun,Lingyun Hu,Yimin Wen,Keming Chen,Zhenjuan Sun,Haiyuan Yue,Chao Zhang..Adenosine triphosphate promotes locomotor recovery after spinal cord injury by activating mammalian target of rapamycin pathway in rats [J].中国神经再生研究（英文版）,2013,8(2):101-110,10.
