首页|期刊导航|中国神经再生研究（英文版）|Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
中国神经再生研究（英文版）2013，Vol.8Issue(2)：156-161,6.DOI:10.3969/j.issn.1673-5374.2013.02.008
Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system
摘要
Abstract
Apolipoprotein C2 is an important member of the apolipoprotein C family, and is a potent activator of lipoprotein lipase. In the central nervous system, apolipoprotein C2 plays an important role in the catabolism of triglyceride-rich lipoproteins. Studies into the exact regulatory mechanism of mouse apolipoprotein C2 expression have not been reported. In this study, seven luciferase expression vectors, which contained potential mouse apolipoprotein C2 gene promoters, were constructed and co-transfected with pRL-TK into HEK293T cells to investigate apolipoprotein C2 promoter activity. Luciferase assays indicated that the apolipoprotein C2 promoter region was mainly located in the +104 bp to +470 bp region. The activity of the different lengths of apolipoprotein C2 promoter region varied. This staggered negative-positive-negative arrangement indicates the complex regulation of apolipoprotein C2 expression and provides important clues for elucidating the regulatory mechanism of apolipoprotein C2 gene transcription.关键词
neural regeneration/basic research/apolipoprotein C2/promoter/dual-luciferase reporter assay/transcriptional activity/regulatory elements/grants-supported paper/neuroregenerationKey words
neural regeneration/basic research/apolipoprotein C2/promoter/dual-luciferase reporter assay/transcriptional activity/regulatory elements/grants-supported paper/neuroregeneration引用本文复制引用
Zhaoyang Li,Bing Du,Shengyang Li,Xiangchuan Lv,Shenglai Zhou,Yang Yu,Wei Wang,Zhihong Zheng..Cloning and characterization of an apolipoprotein C2 promoter in the mouse central nervous system [J].中国神经再生研究（英文版）,2013,8(2):156-161,6.基金项目
This study was supported by grants from the National Natural Science Foundation of China,No.30770818 （）
a grant from Education Department of Liaoning Province,No.2009s109. （）
