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Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells

Xingrong Yan Liwen Li Fulin Chen Yanhong Yang Wei Liu Wenxin Geng Huichong Du Jihong Cui Xin Xie Jinlian Hua Shumin Yu

中国神经再生研究（英文版）2013，Vol.8Issue(4)：293-300,8.

中国神经再生研究（英文版）2013，Vol.8Issue(4)：293-300,8.DOI:10.3969/j.issn.1673-5374.2013.04.001

Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells

Xingrong Yan ¹Liwen Li ¹Fulin Chen ¹Yanhong Yang ²Wei Liu ¹Wenxin Geng ¹Huichong Du ¹Jihong Cui ¹Xin Xie ¹Jinlian Hua ³Shumin Yu⁴

作者信息

1. College of Life Sciences, Northwest University, Xi'an 710069, Shaanxi Province, China
2. Department of Obstetrics & Gynecology, Tangdu Hospital, the Fourth Military Medical University of Chinese PLA, Xi'an 710038, Shaanxi Province, China
3. College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi Province, China
4. College of Veterinary Medicine, Sichuan Agricultural University, Yaan 625001, Sichuan Province, China
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摘要

Abstract

Parthenogenetic embryonic stem cells have pluripotent differentiation potentials, akin to fertilized embryo-derived embryonic stem cells. The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells. Before differentiation, karyotype analysis was performed, with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells. Sex chromosomes were identified as XX. Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene, Oct4, at both the mRNA and protein levels, indicating pluripotent differentiation potential of the two embryonic stem cell subtypes. Embryonic stem cells were induced with retinoic acid to form embryoid bodies, and then dispersed into single cells. Single cells were differentiated in N2 differentiation medium for 9 days. Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin, βIII-tubulin and myelin basic protein. Quantitative real-time PCR found expression of neurogenesis related genes (Sox-1, Nestin, GABA, Pax6, Zic5 and Pitx1) in both types of embryonic stem cells, and Oct4 expression was significantly decreased. Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells. Thus, our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells.

关键词

neural regeneration/ stem cells/ parthenogenesis/ parthenogenetic embryonic stem cells/ embryonic stem cells/ neuronal cells/ karyotypes/ Oct4/ differentiation/ embryoid body/ mice/ grants-supported paper/ photographs-containing paper/ neuroregeneration

Key words

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Xingrong Yan,Liwen Li,Fulin Chen,Yanhong Yang,Wei Liu,Wenxin Geng,Huichong Du,Jihong Cui,Xin Xie,Jinlian Hua,Shumin Yu..Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells[J].中国神经再生研究（英文版）,2013,8(4):293-300,8.

基金项目

This work was supported by the National Natural Science Foundation of China,No.30900155 and 81070496 （）

the Research Foundation of Education Bureau of Shaanxi Province,China,No.09JK785 （）

Foundation of Interdisciplinary for Postgraduates from Northwest University,No.08YJC22 and the Key Laboratory Funding of Northwestern University,Shaanxi Province in China. （）

中国神经再生研究（英文版）

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ISSN：1673-5374

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