中国神经再生研究（英文版）2013，Vol.8Issue(4)：313-319,7.DOI:10.3969/j.issn.1673-5374.2013.04.003
Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold
Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold
Fei Huang 1Qiang Shen 2Jitong Zhao2
作者信息
- 1. Department of Orthopedics, Fourth Affiliated Hospital of Anhui Medical University, Hefei 230000, Anhui Province, China
- 2. Department of Orthopedics, Shanghai First People's Hospital, Shanghai Jiao Tong University, Shanghai 200080, China
- 折叠
摘要
Abstract
Collagen protein is an ideal scaffold material for the transplantation of neural stem cells. In this study, rat neural stem cells were seeded into a three-dimensional collagen gel scaffold, with suspension cultured neural stem cells being used as a control group. Neural stem cells, which were cultured in medium containing epidermal growth factor and basic fibroblast growth factor, actively expanded and formed neurospheres in both culture groups. In serum-free medium conditions, the processes extended from neurospheres in the collagen gel group were much longer than those in the suspension culture group. Immunofluorescence staining showed that neurospheres cultured in collagen gels were stained positive for nestin and differentiated cells were stained positive for the neuronal marker βIII-tubulin, the astrocytic marker glial fibrillary acidic protein and the oligodendrocytic marker 2',3'-cyclic nucleotide 3'-phosphodiesterase. Compared with neurospheres cultured in suspension, the differentiation potential of neural stem cells cultured in collagen gels increased, with the formation of neurons at an early stage. Our results show that the three-dimensional collagen gel culture system is superior to suspension culture in the proliferation, differentiation and process outgrowth of neural stem cells.关键词
neural regeneration/ stem cells/ neural stem cells/ collagen gel/ scaffold/ central nervous system/ proliferation/ differentiation/ neurosphere/ photographs-containing paper/ neuroregenerationKey words
neural regeneration/ stem cells/ neural stem cells/ collagen gel/ scaffold/ central nervous system/ proliferation/ differentiation/ neurosphere/ photographs-containing paper/ neuroregeneration引用本文复制引用
Fei Huang,Qiang Shen,Jitong Zhao..Growth and differentiation of neural stem cells in a three-dimensional collagen gel scaffold[J].中国神经再生研究（英文版）,2013,8(4):313-319,7.
